我们前期通过数据挖掘发现，气阴两虚和热毒炽盛是白血病最主要的证候，益气养阴解毒为其基本治则。前期研究表明益气养阴解毒中药能够改善白血病患者的临床症状，提高生活质量，延长生存期，并可通过线粒体途径诱导人白血病细胞凋亡。线粒体是代谢通路的中心和细胞凋亡的主要通路。线粒体结构处于融合与分裂的动态平衡中，若动态失衡则线粒体功能异常，在细胞凋亡早期引发自噬。基于此，我们提出假说：益气养阴解毒中药可以通过线粒体融合/分裂途径调控线粒体自噬诱导白血病细胞凋亡。本课题拟通过建立NOD/SCID白血病小鼠模型，结合基因转染技术，检测益气养阴解毒中药作用下白血病细胞线粒体融合/分裂相关蛋白、自噬相关因子、线粒体DNA以及凋亡相关因子等的差异表达，探索靶向调控线粒体动态平衡在白血病细胞自噬与凋亡过程中的作用，为传统医学的创新和发展，以及中西医结合治疗血液肿瘤疾病提供理论依据。

Previous data mining found that Qi and Yin deficiency syndrome and heat toxicity stagnation syndrome are the most common syndrome types of leukemia, whose basic rule is Supplementing Qi, Nourishing Yin and Detoxification. Our previous studies have confirmed that Chinese medicine of Yiqi Yangyin Jiedu can replenish Qi, eliminate evil and detoxify, which can improve the clinical symptoms of leukemia patients, improve the quality of life, prolong the survival period, and induce apoptosis of human leukemia cells through mitochondrial pathway. Mitochondria are central to metabolic pathways and the main apoptotic pathway. The mitochondrial structure is in the dynamic balance of fusion and fission. If the dynamic imbalance occurs, the mitochondrial function is abnormal, and autophagy is triggered in the early stage of cell apoptosis. Based on this, we propose a hypothesis: Yiqi Yangyin Jiedu Chinese medicine can induce apoptosis of leukemia cells by mitochondrial autophagy through mitochondrial fusion/fission pathway. In this study, we intend to establish NOD/SCID leukemia mice model and detect the differential expression of mitochondrial fusion/fission-related proteins, autophagy-related factors, mitochondrial DNA and apoptosis-related factors in leukemia cells under the action of Yiqi Yangyin Jiedu Chinese medicine, and explore the role of targeted regulation of mitochondrial dynamic balance in the process of autophagy and apoptosis of leukemia cells. The innovation and development of traditional medicine and the combination of traditional Chinese and Western medicine in the treatment of hematological tumors provide theoretical basis.