基于活性成分群的肠吸收转运机制多维解析黄芩道地药材的“质-效”本质

道地性是千百年来人们评价中药品质的独特的、综合性的“金标准”。道地药材物质基础本质问题一直是制约道地药材发展的“瓶颈”问题。道地药材想要长远、科学的发展，必须开展多学科交叉和交流，基于“道地药材的核心物质基础是组分之间独特的比例”的假说，从“活性成分群—肠吸收转运—药效”开展多维关联性研究，构建道地药材的“质-效”评价体系。本研究以代表性道地药材黄芩为示范对象，选择体现黄芩传统功效的药理指标，结合Caco-2细胞模型研究道地与非道地药材中活性成分群的肠吸收转运机制，采用色谱和质谱技术，逐步解析黄芩水提物中对肠吸收转运和药效综合贡献度大的组分。同时借助效应面设计，对不同比例组分进行组合筛选，还原并整合出疗效最佳的“黄酮优化组分”，进行验证实验。本研究目的，一方面为阐明黄芩道地药材“质优效佳”的物质基础本质内涵提供依据，另一方面为道地药材物质基础研究提供新思路。

Genuineness is a unique, comprehensive standard, which is used to evaluate the quality of traditional Chinese medicines for thousands of years. The essence problem of the material basis of the geoherbs is the bottleneck problem and has restricted the development of geoherbs, therefore, a long-term and scientific development must involve a multidisciplinary cooperation. Based on the hypothesis that the core material basis of the geoherbs is the unique ratio between the components in TCMs , we will carry out multi-dimensional association studies, and build the evaluation system of quality and efficacy for the geoherbs from three respects, including active ingredients, intestinal absorption and efficacy . Therefore, Scutellariae Radix will be investigated as a representative object. Pharmacological indices closely related to traditional efficacy will be studied. Caco-2 cell models will be used to study the mechanism of intestinal absorption of the active ingredients in geoherbs and non-geoherbs. And then the research will gradually analyze the components in the aqueous extract of Scutellariae Radix by chromatography and mass spectrometry technologies and find these components with the largest contribution to efficacy and intestinal absorption. At the same time the components with different proportions will be screened by the response surface designs, and the optimal flavonoid components with the best efficacy will be restored, integrated, and verified. The purpose of this study is to provide experimental data to clarify the essential connotation of the material basis of the geoherbs on one hand, and to provide a new idea for researching the material basis of the geoherbs on the other hand.