Response Surface Optimized Infrared-Assisted Extraction and UHPLC Determination of Flavonoid Types from Flos Sophorae.
Response Surface Optimized Infrared-Assisted Extraction and UHPLC Determination of Flavonoid Types from Flos Sophorae.
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响应面优化红外辅助提取及 UHPLC 测定槐花中黄酮类型
DOI：
10.3390/molecules22061000
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发表时间：
2017-06-15
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Mou Q;He J;Yin R;Yang B;Fu M;Fang J;Li H
采用单因素试验和Box-Behnken设计优化红外辅助提取槐花中芦丁、槲皮素、山萘酚和异鼠李素的工艺条件。研究了提取溶剂、料液比、提取时间、红外功率4个因素对黄酮提取率的影响。在优化条件下，提取率为33.199 ± 0.24 mg/g，与加热回流提取（HRE）和超声波辅助提取（UAE）相比有了显著提高，提取时间仅为9 min，洗脱液中富含2，2-二苯基-1-苦肼基（DPPH）和2，2 ′-偶氮双清除DPPH自由基的IC_（50）为53.44 ± 0.01 μg/mL，氧自由基吸收容量（ORAC）为3785.83 ± 52 μmol/g，均高于HRE法和UAE法。此外，还优化了超高效液相色谱法用于黄酮类化合物的定性和定量分析，并通过相关系数（r）、重现性（RSD，n = 5）和回收率（r = 0.9994-0.9998，RSD = 0.74- 1.83%，n = 5）对方法进行了验证。这些结果表明，高提取率的黄酮类化合物的结果，更强的抗氧化价值和响应面优化的IRAE是一个有前途的替代传统的提取技术，从药用植物黄酮类化合物。
Single-factor experiment and Box-Behnken design were applied to optimize the infrared-assisted extraction (IRAE) of rutin, quercetin, kaempferol, and isorhamnetin from Flos sophorae. Four factors (extract solvent, solid-liquid ration, extraction time, infrared power) affecting the extraction yield of flavonoids were studied. Under optimized conditions the extraction yield was 33.199 ± 0.24 mg/g, which substantially improved, compared with heating reflux extraction (HRE) and ultrasonic-assisted extraction (UAE), while extraction time was only 9 min. The eluents were rich in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azobis (2-methyl-propionamidine) dihydrochloride radical scavenging potential (IC50 of DPPH: 53.44 ± 0.01 μg/mL, oxygen radical absorbance capacity (ORAC): 3785.83 ± 52 μmol/g) than the extracts obtained by HRE and UAE. In addition, an ultra-high performance liquid chromatography method was optimized for the identification and quantification of the tested flavonoids, and the method was validated based on its correlation coefficient (r), reproducibility (RSD, n = 5), and recovery values, which were 0.9994–0.9998, 0.74–1.83%, and 97.78–102.94%, respectively. These results confirmed that high extraction yield of flavonoids results in stronger antioxidant values and response surface methodology optimization of IRAE is a promising alternative to traditional extraction techniques for flavonoids from medicinal plants.
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