西藏野生大麦相较于普通栽培大麦蕴藏黑色籽粒种质资源，含更多多酚类黄酮物质，具有十分珍贵的保健作用。然而黑色籽粒的控制基因与遗传模式如何却未知。本课题组前期已将大麦黑色籽粒关键基因（blp）定位在1H染色体长臂端的17kb区间内，比对大麦全基因组序列信息仅有3个候选基因。本研究拟在前期研究基础上对候选基因进行大群体重测序以进行候选基因关联分析筛选blp基因；进一步对该候选基因进行转基因验证，明确控制黑色籽粒性状的关键基因。同时，明确黑色籽粒中的黑色特征物质及blp基因如何调控黑色籽粒的形成；了解西藏野生大麦中黑色籽粒形成与青藏高原地区高紫外辐射环境的关系。这一项目的成功实施将为揭示大麦黑色籽粒性状的分子遗传机制和培育黑色大麦新品种奠定基础。

Compared to the yellow grain of cultivated barley, the black grain germplasm in Tibetan wild barley has distinguished values, which contains high content of polyphenols and flavonoids. However, it is unclear about the genetic mechanism and controlling genes of black barley formation. In our previous study, it has been found that the black lemma and pericarp formation of barley was controlled by blp gene, which was located in a 17kb region at the long arm of chromosome 1H. Compared to the whole genome sequence information, there were 3 genes in the 17kb candidate region. In this project, the key gene of blp will be identified based on the 3 candidate genes association analysis in the Tibetan wild barley population. Furthermore, transgenic work will be done to confirm the function of blp gene. Moreover, the chemical constituent of black pigment and the mechanism of blp gene regulated the black pigment formation will be studied. The relationship between black barley formation and the high UV radiation environment in the Tibet plateau will also be investigated. It may be expected that the results can reveal the genetic mechanism of black barley formation and provide theoretical guidance of black barley cultivars breeding.