植物真菌侵染结构的形成与扩展对侵染性病害的发生起着极其重要的作用。植物锈菌是活体营养专性寄生菌，在其侵染过程中发现了一类侵染结构分化基因INFs，初步研究表明该类基因为锈菌特有的。然而，由于锈菌不能进行人工培养和稳定的遗传转化，加之病菌基因组复杂，目前对该类锈菌特异INF基因介入的致病机理知之甚少。本项目以小麦重要病害致病真菌-小麦条锈菌为研究对象，借助菜豆锈菌已鉴定的INF类基因，从全基因组水平鉴定并克隆获得小麦条锈菌INF类同源基因；利用实时定量RT-PCR分析基因表达特征，明确基因的亚细胞定位及蛋白分布特征；进一步通过筛选酵母双杂交cDNA文库获得其靶标蛋白基因；最终采用HIGS技术解析基因的功能。本研究将为揭示小麦条锈菌INF类基因的致病分子机理，及建立小麦条锈病持久控制的新策略提供基因资源和奠定理论基础。

During infection process of plant fungi, formation and development of infection structures is vital for fungi to infect plant successfully. Rust fungi belong to obligate biotrophic pathogen. Previous studies reported a set of infection-structure (INF) genes and indicated INF genes are rust fungi-specific. However, due to the lack of reliable transformation and gene disruption method, knowledge about the function of INF genes involved in pathogenesis is limited. In this study, we aim to identify INF genes in wheat stripe rust fungus, Puccinia striiformis f.sp. tritici (Pst) in genome-wide. Real time RT-PCR was used to analyze transcript profiling of INF genes.Subcellular localization of INF genes were also investigated. Moreover, INF-interacting genes were obtained by screening estalbished yeast two-hybrid cDNA library. Finally we use HIGS technique to perform functional analysis of these genes. Through performing this project we will reveal pathogenesis of this rust fungi-specific INF genes in Pst and it will provide theoretical basis and gene resources for establishing a novel strategy for sustainable control of wheat stripe rust.