条锈菌作为专性寄生真菌，毒性变异频繁，其引起的小麦条锈病严重威胁我国小麦生产安全。采用使感病基因丧失功能的育种策略进行育种，速度较快且所育品种具有广谱和持久抗病特点。类钙调磷酸酶B蛋白（CBLs）与其互作蛋白激酶（CIPKs）组成的CBL-CIPK信号途径在植物非生物胁迫及生物胁迫等方面发挥重要作用。然而，CBL-CIPK信号途径在植物，特别是禾谷类作物中的感病机制尚不明晰。通过前期研究，我们从CBL-CIPK信号途径中获得了一个重要候选感病基因TaCIPK14。本项目拟在前期研究基础上，明确TaCIPK14的感病功能，分析其激酶活性与小麦感病性的关系，探明钙信号和TaCBLs对其激酶活性的调控作用，最终鉴定其下游靶标蛋白并解析调控机理。本研究将阐明TaCIPK14的感病功能及其调控小麦免疫反应的作用机理，并为小麦条锈病持久控制奠定理论基础。

Puccinia striiformis f. sp. tritici (Pst), as an obligate biotrophic fungus, causes destructive wheat stripe rust in China. Generating and exploiting a genetic loss of susceptibility is one of new strategies in breeding and biotechnology are required to obtain durable disease resistance. Calcineurin B-like proteins (CBLs) interact with their protein kinases (CIPKs), and the CBL-CIPK signaling pathway plays important roles in abiotic stress and biotic stress in plants. However, the molecular mechanism of CBL-CIPK signaling pathways in plant susceptibility, especially in cereal crops, remains unclear. In our previous studies, we identified an important candidate susceptibility gene TaCIPK14 in wheat. In this project, we will verify the susceptibility function of TaCIPK14, analyze the kinase activity of TaCIPK14 and its role during interaction between wheat and Pst, define the kinase activity of TaCIPK14 regulated by Ca2+ and TaCBLs, identify the downstream target proteins of TaCIPK14 and illuminate the regulation mechanism. This project will reveal the susceptibility role of TaCIPK14 in regulating host defense and provide theoretical basis for sustainable control of wheat stripe rust.