多巴胺受体1通过调控lncRNAs表达调节飞蝗嗅觉吸引行为的机理研究

多巴胺受体1（Dop1）调节飞蝗群聚中的嗅觉吸引行为，长链非编码RNA （lncRNAs）调控多巴胺信号传递。但是Dop1通过调控lncRNAs表达调节飞蝗嗅觉吸引行为的神经分子机制不清楚。采用高通量测序分析受Dop1影响并在型变中表达差异的lncRNAs后，筛选受Dop1调控并与嗅觉行为相关的lncRNAs。在验证候选lncRNAs对嗅觉行为调节以及与Dop1的功能联系后，分析lncRNAs的组织定位、非编码特性以及克隆lncRNAs的启动子。通过RNA和DNA pulldown、RNA免疫共沉淀、GST pull down、凝胶阻滞实验以及荧光素酶报告基因等方式验证候选蛋白与lncRNA及其启动子的相互作用，分析Dop1是否通过候选蛋白影响lncRNAs的表达，并分析lncRNA影响下游基因的表达谱，以期初步回答Dop1-lncRNAs-靶基因环路调节嗅觉吸引行为的神经分子机制。

Dopamine receptor 1 (Dop1) mediates olfactory attraction during the aggregation of migratory locusts, but it is unkown that Dop1 mediates the expression of long non-coding RNA(lncRNA) underlying olfactory attraction. Here, we apply high throughput sequencing to analyze the expression of lncRNAs followd by the activation of Dop1 by agonists and inhibition of Dop1 by RNAi knock down. We also analyze the expression patterns of lncRNAs during phase change of the migratory locust. After we confirm that lncRNAs candidate mediates olfactory attraction and their correlation with Dop1 underlying olfactory response, we analyze the location of lncRNAs in cells, its coding potential and activation of lncRNAs promoter. Then we apply RNA and DNA pull down, RNA immunoprecipitation, GST pull down, EMSA, and lucifererase reporter system to confirm the interaction between lncRNAs and their RNA binding proteins. We also analyze whether Dop1 signaling mediates binding of candidate protein with lncRNAs. Moreover, we analyze gene expression profiles after lncRNA knockdown. In this study, we hope to confirm the Dop1-lncRNA-target circuit that mediates neurophysiological mechanism underlying olfactory attraction.