细胞周期调控机制是当前肿瘤细胞生物学研究的热点之一。已有的研究显示，上皮剪接调节蛋白-1（ESRP1）具有抑制肿瘤细胞上皮间质转换（EMT）的作用，但其对肿瘤细胞周期的调控作用及机制还未见报道。我们最近的研究发现，过表达ESRP1可下调细胞周期素A2（CyclinA2）表达并抑制子宫颈癌细胞由G1期向S期转换，即发生G1阻滞。本课题将在这一发现基础上，拟以临床子宫颈癌肿瘤标本、裸鼠荷瘤模型及体外培养的子宫颈癌细胞系作为研究对象，利用细胞分子生物学、小动物活体成像及免疫组化等技术，深入探讨ESRP1对肿瘤细胞周期的调控作用。阐明其通过结合CyclinA2 mRNA的3’UTR区，在转录后水平下调其表达并诱导子宫颈癌细胞发生G1期阻滞进而抑制肿瘤细胞增殖的分子机制。研究结果将揭示细胞周期调控网络的新机制，并为肿瘤防治提供新思路和理论依据。

Molecular mechanism of cell cycle control is a popular topic in tumor study. It has been reported that ESRP1 can inhibit the ESRP1and plays an important role in tumor metastasis, but it is still not cler whether ESRP1 has direct influences on cell cycle and possible molecuar mechanisms. In our previous work, we demonstrated that ESRP1 can down-regulate the expression of cyclin A2 and induce cervical cancer cell cycle arrest at the G1 phase. Based on our previous works, we try to investigate the effects of ESRP1 on tumor cell cycle regulation using molecular and cell biology methods (including immunoblot, RNAi, western blot, flow cytometry and RIP, etc) under in vitro or in vivo conditions. The study will reveal the mechanism of cell cycle regulation, and will be contribute to further understand the law of tumor cell proliferation, and provide new ideas and theoretical basis for prevention and cure of malignanttumor.