红苞凤梨叶片因花青素、叶绿素、类胡萝卜素时空动态积累而出现绿、黄、白、红相间的嵌合叶色，是研究色素竞争积累规律的理想材料。色素协同竞争积累调控机理的研究对深入解析嵌合叶色形成机理具有重要意义。申请人前期研究揭示了金边嵌合叶片叶绿素时空差异代谢积累的调控机理。本项目将在前期研究基础之上，以不同呈色特征的红苞凤梨叶色嵌合体为材料，弄清三种色素时空动态积累规律及其对嵌合叶色呈现的决定作用机制。通过Y1H、Y2H、非编码RNA预测分析，鉴定色素代谢的调控因子，构建代谢调控分子网络。在三类色素代谢调控网络的交叉联系中筛选色素协同竞争积累的关键调控因子；通过Y2H、BiFC、qPCR、双荧光素酶分析、遗传转化等验证关键调控因子功能。从转录调控、蛋白质调控、非编码RNA表观调控水平研究色素协同竞争积累的调控机理，探索红苞凤梨叶色嵌合体的形成机理，为嵌合性状的分子改良和稳定性调控提供基础。

The dynamic accumulation of anthocyanins, chlorophyll and carotenoids resulted in the colorful leaf character of Ananas comosus var. bracteatus. It is very important to analyze the rule and regulation mechanism of synergistic competitive accumulation of different pigments for the revealing of formation mechanism of chimeric leaf color. Our previous studies revealed the regulatory mechanism of spatial-temporal different metabolism and accumulation of chlorophyll in chimeric leaves. Based on our previous studies, the application project will study on the regulatory mechanism of synergistic competitive accumulation relationship between anthocyanins, chlorophyll and carotenoids. Three different types of leaf chimera are used as materials in this research. To clarify the spatiotemporal dynamic accumulation rules of three pigments and the determining mechanism of their effect on the color presentation of chimeric leaves. Identify the regulatory factors of pigment metabolism and construct the regulatory molecular network by Y1H, Y2H and non-coding RNA predictive analysis. To screen the key regulatory factors by analyze the cross link among the regulatory networks of three kinds of pigment. Using Y2H, BiFC, real time qPCR, dual-luciferase assay, genetic transformation to identify the function of the key regulatory factors. The regulatory mechanism of pigment synergistic competition accumulation was studied from the perspectives of transcriptional regulation, protein regulation and non-coding RNA epigenetic regulation to explore the forming mechanism of Mosaic leaf color of bromeliads rosea. The research results could provide a basis for molecular improvement and stability regulation of chimeric traits.