红苞凤梨有金边和金心两种嵌合类型，花果艳丽、观赏期长，是重要的新型观赏植物。同一嵌合类型叶片嵌合方式多样，白色条纹数量、宽度和形状都不一致，且嵌合性状易发生变异甚至消失。深入研究叶片嵌合性状的形成机理具有重要意义。申请人前期研究揭示了其叶片白化失绿突变机理，筛选出突变关键基因（POR）。本项目将以金边和金心嵌合植株为材料，分析顶端分生组织分化发育成叶片的组织细胞学过程；以POR为探针进行RNA-原位杂交，研究绿、白细胞在顶端分生组织中的分布形式及其对叶原基嵌合类型的决定作用机制，再进一步研究叶原基发育成叶片过程中绿、白细胞的生长速度、数量和分布及其对叶片条纹嵌合方式的影响。采用高通量测序探索白色条纹嵌合方式形成的分子机制。探明红苞凤梨金边和金心嵌合类型形成的组织细胞学起源，揭示叶片嵌合方式形成机理及同一嵌合类型白色条纹数量、宽度和形状的调控机制，为嵌合性状的分子改良和稳定性调控奠定基础。

Variegated chimera varieties of Ananas comosus var. bracteatus have been planted as landscape plants for its ornamental values of color leaf and fruit. The number, width and shape of the white stripe of the leaves of same chimeric type are different. However the variegated chimera character of bromeliad is unstable. Many plants regenerated by in vitro tissue culture lost ornamental value for the losing of leaf color. It is very important and meaningful to study the formation mechanism of the chimera character. Our studies revealed the chlorosis mechanism of the albino cells of Ananas comosus var. bracteatus, and confirmed that the POR gene is the key gene plays role in the chlorosis. Based on our preliminary studies, the gilt-edged and gilt-middle plant were used to analyze histocytology procedure of the formation of leaf primordium and the development of leaf; Using POR gene as probe to analyze how the distribution of the green and white cells in SAM decide the chimeric type of the leaf primordium; Study the effects of growth rate, number and distribution of the white cells on the chimeric mode of the white stripe; The formation molecular mechanism of the chimeric mode of the white stripe was studied by high throughput sequencing. This study could illuminate the histocytology origination of the chimeric type of the leaf primordia and the formation mechanism of the chimeric mode of the leaf. The research results could lay a foundation of the molecular improvement and stability regulation of the chimera character.