近年来，新城疫病毒（NDV)感染致病机制引起科学家的广泛关注，但是从宿主信号通路变化而诱导的固有免疫反应对NDV感染致病机制的研究较少。本项目通过应用qPCR、RNAi、激光共聚焦扫描、凝胶阻滞和Pull down等技术研究病毒感染后细胞NF-κB和IRF7信号通路的变化及该变化对NDV复制的影响。构建NDV V和W基因真核过表达系统和应用反向遗传技术获得V/W基因缺失NDV 毒株，结合构建的报告基因研究NDV V/W蛋白对细胞NF-κB和IRF7信号通路的调控及对NDV复制的影响。在此基础上，对NDV V/W C末端进行分段，应用Pull down 和点突变技术确定影响NF-κB和IRF7信号通路的NDV V/W C末端核心区域序列和关键核苷酸。本项目的实施为宿主固有免疫反应影响NDV的复制和NDV逃逸宿主固有免疫反应的致病机制奠定理论基础，为病毒进化研究以及疫苗研发提供科学的指导依据。

Many scientists have payed close attention to the infection mechanism of causing disease by Newcastle disease virus(NDV) over recent years. But there is little information in the papers about the effect of the host innate immunity from the change of the signal transduction pathways to NDV infection mechanism of causing disease.This research will study the effect of NDV replication by various types of NF-κB and IRF7 signal transduction pathways after NDV infecting CEF by many technologies including qPCR,RNAi,immunofluorescence assay,electrophoretic mobility shift assay and Pull down et al. By combining Ch-IRF7-Luc, Ch-NF-κB-Luc and Ch-STAT1-Luc reporter genes,to study of NDV V/W proteins on the NF-κB and IRF7 signal transduction pathway regulation and on NDV replication from the over-expression of NDV V / W protein and V/W gene deletion NDV strains infection of host.The accomplishment of this research will establish theoretical foundation for innate immunity interfering with NDV replication and the mechanism of causing a disease of NDV escaping host innate immunity.It will provide the guidance of a scientific theory for the research of virus evolution, vaccine development and clinical application.