有益微生物的互作对植物生长抗逆的协同增效作用是当前国际研究的热点。当前林业苗圃生产中连作障碍导致的问题主要包括：土传病害发生严重、土壤微生态环境破坏、苗木产量和品质下降，亟待开发环境和谐性微生物修复剂来克服连作障碍、控制病害发生、提高苗木质量。外生菌根菌褐环乳牛肝菌及其菌根辅助细菌是樟子松重要的根际微生物类群，在提高苗木抗逆性、修复土壤微生态环境方面发挥重要作用。本项目在前期研究的基础上，通过诱导分离和筛选褐环乳牛肝菌菌根辅助细菌，探讨菌根辅助细菌对菌根菌生长及对病原菌抑制作用机理，利用生物化学分析技术研究褐环乳牛肝菌-菌根辅助细菌-樟子松体系对病原菌生物胁迫的响应模式，阐明褐环乳牛肝菌-菌根辅助细菌互作对樟子松促生抗病机制，为进一步研制对植物具有促生抗逆作用的微生物菌剂提供理论基础。

The synergistic interaction of some beneficial microbiology on improving host growth and adverse resistance is an international research focus.The main problems in forestry nursery caused by continuous cropping obstacle include: disease occurred seriously, Soil physical and chemical environmental damage, decline of seedling yield and quality. It is necessary to research environmental harmony and bioremediation agent to control soil-borne disease and overcome continuous cropping obstacle. Ectomycorrhizal fungi Suillus luteus and its mycorrhizal helper bacteria are two important rhizosphere microorganism groups of conifers, and have some positive contributions to host include control soil-borne disease and enchance host enviroment tolerance. After induced isolate and screen mycorrhizal helper bacteria of Suillus luteus,to research the in vivo interaction between Suillus luteus, mycorrhizal helper bacteria and Rhizoctonia solani, and the biochemical analysis technology was employed to research the physiological response of 'Suillus luteus-mycorrhizal helper bacteria-pinus' system after induced by Rhizoctonia solani, clear and definite the mechanisms of synergistic interaction between Suillus luteus and mcorrhizal helper bacteria on improving pine seedlings growth and stress resistance in the end. The research progress of this project can provide theoretical basis to develop the plant promote and stress resistance microbial agent.