碱性螺旋环螺旋（bHLH）蛋白是一类调控植物发育及抗逆基因表达的重要转录因子。前期我们从盐生植物灰绿藜中发现了一个bHLH转录因子-Cg bHLH001，与拟南芥bHLH因子的XII亚族关系最近，涉及发育、逆境响应等功能。然而Cg bHLH001在序列和结构上与XII亚族成员存在明显差异，且XII亚族在植物抗逆中的调控机制报道十分有限。基于此，本研究以灰绿藜Cg bHLH001转录因子为材料，拟通过qPCR和免疫印迹法分析其发育及胁迫表达模式；通过构建N端和C端系列缺失片段克隆确定蛋白互作和转录激活功能域；同时通过染色质免疫共沉淀及大规模测序手段鉴定转录因子在植物体内结合的DNA位点信息；最后通过转基因及转录组数据分析Cg bHLH001下游调控基因及其抗逆功能。通过上述结果，阐明灰绿藜bHLH转录因子的调控机制及抗逆功能，揭示“结构-调控-功能”的分子基础，为抗逆分子育种提供科学依据。

Basic helix-loop-helix(bHLH) protein is an important transcription factor (TF) in regulation of gene expression of plant development and stress tolerance . In previous work, we revealed a bHLH TF-Cg bHLH001 in halophyte Chenopodium glaucum, which shared relatively higher homology to Arabidopsis XII bHLH TF, and was involved in functions of plant development and stress responses. However, distinct differences in the sequence and protein domain exist between Cg bHLH001 and the members of XII TF subfamily, and the regulation mechanism of XII TF subfamily in stress tolerance remains largely unclear. Based on this, in the present study, we employed Cg bHLH001 TF as material, try to analyze the expression patterns of Cg bHLH001 TF in plant development and stress responses by quantitative RT-PCR and Western blot; to identify the N-terminal and C-terminal key functional domains for protein interaction and/or transcriptional activation by truncated fragments; meanwhile, to determine the DNA binding sequence of Cg bHLH001 TF in vivo by chromatin immuno-precipitation (ChIP) and high throughput sequencing (seq); finally, to analyze the stress tolerance function of Cg bHLH001 TF and its regulation of the downstream genes. By fulfilling the above targets, we try to clarify the regulation mechanisms of Cg bHLH001 and its function in stress tolerance, which reveals a molecular basis pattern as “structure-regulation-function”, and can also provide scientific evidence for molecular breeding in development of stress tolerance crops.