The bHLH transcription factor CgbHLH001 is a potential interaction partner of CDPK in halophyte Chenopodium glaucum.
The bHLH transcription factor CgbHLH001 is a potential interaction partner of CDPK in halophyte Chenopodium glaucum.
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bHLH 转录因子 CgbHLH001 是盐生植物灰绿藜中 CDPK 的潜在相互作用伙伴
DOI：
10.1038/s41598-017-06706-x
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发表时间：
2017-08-16
影响因子：
4.6
通讯作者：
Lan H
中科院分区：
文献类型：
--
作者：
Wang J;Cheng G;Wang C;He Z;Lan X;Zhang S;Lan H
植物进化出了不同的能力，以适应不断变化的环境以适应植物的生长。钙依赖蛋白激酶(CDPK)被认为在非生物应激信号中起着关键作用。到目前为止，对CDPK在逆境中识别的特定底物的研究还很有限。在本研究中，我们揭示了在盐胁迫下，CDPK和一个bHLH转录因子之间的潜在相互作用。首先，我们鉴定了一个在盐和干旱胁迫下上调的CgCDPK；然后通过Y2H筛选，发现CgCDPK参与了与bHLHTf(命名为CgbHLH001)的相互作用，该Tf对盐和干旱胁迫也有正向反应。进一步的计算预测和GST-Pull和BIFC分析表明，CgCDPK和CgbHLH001之间存在潜在的相互作用，它们可能在质膜上相互作用。此外，CgCDPK过表达的转基因烟草株系还能显著积累NtbHLH(与烟草CgbHLH001同源基因)的转录产物，这为CgCDPK与CgbHLH001的相关性提供了又一证据。我们的结果表明，CgbHLH001在响应非生物胁迫的信号转导通路中可以与CgCDPK相互作用，这将为进一步了解植物CDPK信号通路的底物特异性提供新的证据。
Plants have evolved different abilities to adapt to the ever-fluctuating environments for sessility. Calcium-dependent protein kinase (CDPK) is believed to play a pivotal role in abiotic stress signaling. So far, study on the specific substrates that CDPK recognized in response to adversity is limited. In the present study, we revealed a potential interaction between CDPK and a bHLH transcription factor under salt stress in Chenopodium glaucum. First, we identified a CgCDPK, which was up-regulated under salt and drought stress; then by Y2H screening, CgCDPK was detected to be involved in interaction with a bHLH TF (named as CgbHLH001), which also positively respond to salt and drought stress. Further computational prediction and experiments including GST-pulldown and BiFC assays revealed that potential interaction existed between CgCDPK and CgbHLH001, and they might interact on the plasma membrane. In addition, CgCDPK-overexpressed transgenic tobacco line could significantly accumulate transcripts of NtbHLH (a homolog of CgbHLH001 in N. tabacum), which provided another evidence of correlation between CgCDPK and CgbHLH001. Our results suggest that CgbHLH001 can interact with CgCDPK in signal transduction pathway in response to abiotic stress, which should provide new evidence for further understanding of the substrate specificity of plant CDPK signaling pathway.
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影响因子：
20.5
作者：
Boudsocq, Marie;Sheen, Jen
通讯作者：
Sheen, Jen
影响因子：
4.3
作者：
Chen, Yuan;Li, Fei;Xu, Yunyuan
通讯作者：
Xu, Yunyuan
DOI：
10.1073/pnas.86.7.2172
发表时间：
1989-04-01
影响因子：
11.1
作者：
CHUNG, CT;NIEMELA, SL;MILLER, RH
通讯作者：
MILLER, RH
影响因子：
5.6
作者：
Horstman A;Tonaco IA;Boutilier K;Immink RG
通讯作者：
Immink RG
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K