Investigation of the Nature of CgCDPK and CgbHLH001 Interaction and the Function of bHLH Transcription Factor in Stress Tolerance in Chenopodium glaucum.
Investigation of the Nature of CgCDPK and CgbHLH001 Interaction and the Function of bHLH Transcription Factor in Stress Tolerance in Chenopodium glaucum.
复制标题
CgCDPK 与 CgbHLH001 相互作用的性质及 bHLH 转录因子在灰藜胁迫耐受中的功能研究
DOI：
10.3389/fpls.2020.603298
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Lan H
中科院分区：
文献类型：
--
作者：
Zhou Z;Wang J;Zhang S;Yu Q;Lan H
钙依赖性蛋白激酶（Calcium-dependent protein kinase， CDPK）及其底物在植物对逆境的响应中起着重要作用。到目前为止，关于CDPK和下游相互作用成分（特别是转录因子，TFs）表征的文献是有限的。在本研究中，CgCDPK（蛋白激酶）（accession no. 1）与植物间的相互作用。MW26306)和CgbHLH001 (TF)(登记号：进一步解剖盐生植物青花藜（Chenopodium glaucum）的MT797813基因。首先，我们发现CgCDPK和CgbHLH001相互作用的可能性质是磷酸化，而CgbHLH001的n端，特别是其内部的第96丝氨酸（潜在的磷酸化位点）是相互作用所必需的，而96Ser突变为丙氨酸并没有改变其核定位，这是由n端和bHLH结构域共同决定的。此外，我们通过烟草中CgbHLH001基因的异位过表达验证了其在逆境胁迫下的功能；转基因系表现出较强的抗逆性，可能是通过改善生理性能和胁迫相关基因表达来实现的。综上所述，本研究表征了C. glaucum中CDPK与bHLH相互作用的生物学意义，并验证了CgbHLH001在胁迫耐受性中的积极作用，为更好地理解CDPK信号通路在逆境中的作用提供了更多证据。
Calcium-dependent protein kinase (CDPK) and its substrates play important roles in plant response to stress. So far, the documentation on the characterization of the CDPK and downstream interaction components (especially transcription factors, TFs) is limited. In the present study, an interaction between CgCDPK (protein kinase) (accession no. MW26306) and CgbHLH001 (TF) (accession no. MT797813) from a halophyte Chenopodium glaucum was further dissected. Firstly, we revealed that the probable nature between the CgCDPK and CgbHLH001 interaction was the phosphorylation, and the N-terminus of CgbHLH001, especially the 96th serine (the potential phosphorylation site) within it, was essential for the interaction, whereas the mutation of 96Ser to alanine did not change its nuclear localization, which was determined by the N-terminus and bHLH domain together. Furthermore, we verified the function of CgbHLH001 gene in response to stress by ectopic overexpression in tobacco; the transgenic lines presented enhanced stress tolerance probably by improving physiological performance and stress-related gene expression. In conclusion, we characterized the biological significance of the interaction between CDPK and bHLH in C. glaucum and verified the positive function of CgbHLH001 in stress tolerance, which may supply more evidence in better understanding of the CDPK signaling pathway in response to adversity.
登录
查看更多内容
影响因子：
5.3
作者：
Feng XM;Zhao Q;Zhao LL;Qiao Y;Xie XB;Li HF;Yao YX;You CX;Hao YJ
通讯作者：
Hao YJ
影响因子：
2.9
作者：
Deng, Cuiyun;Ye, Haiyan;Yan, Jianbin
通讯作者：
Yan, Jianbin
影响因子：
20.5
作者：
Boudsocq, Marie;Sheen, Jen
通讯作者：
Sheen, Jen
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
11.6
作者：
Igarashi, D;Ishida, S;Takahashi, Y
通讯作者：
Takahashi, Y