USC来源的Exosomes复合光敏水凝胶在人工阴道成形术中的作用和机制

人工阴道成形术需要在直肠和膀胱间隙分离的穴道中覆盖内衬材料，往往需要牺牲正常组织。光致亚胺交联水凝胶材料（piGEL）是一种原位成胶后具有优异组织粘附性，并能逐步降解的新型材料，非常适合作为人工阴道内衬材料。同时，本课题组的前期研究发现尿源干细胞外泌体（hUSC-Exos）能很好促进皮肤缺损愈合，表明外泌体可发挥干细胞样作用，可用于无细胞治疗。进一步的研究提示，外泌体作用后的新生组织PI3K/Akt信号通路相关基因明显变化。因此本课题拟利用hUSC-Exos促进创面愈合的能力和piGEL优异的粘附特性，构建hUSC-Exos-piGEL作为理想的人工阴道内衬材料。应用猪阴道壁缺损模型评估原位成胶的hUSC-Exos-piGEL在人工阴道成形术中促进创面愈合及上皮化的效果，重建完美的人工阴道。并进一步阐明hUSC-Exos-piGEL调节PI3K/Akt信号通路促进愈合的分子机制。

Vaginoplasty needs to apply covering/lining material into artificial space between the rectum and bladder by sacrificing autologous normal tissues. Phototriggered-imine crosslinked hydrogel (piGEL) is a new degradable material which has excellent tissue adhesion property and suitable for lining artificial vagina. Furthermore, we found that exosomes of human urine-derived stem cell (hUSC-Exos) could promote the healing of the skin defect and PI3K/Akt signaling pathway related genes in tissue changed in this process. hUSC-Exos could play the role of stem cells and be used as cell-free therapy. Our proposal intends to combine the ability of hUSC-Exos to promote wound healing and excellent tissue adhesion properties of in situ formed piGEL to hUSC-Exos-piGEL. The miniature pig vaginal wall defect model will be used to evaluate the effect of hUSC-Exos-piGEL on wound healing and epithelialization in vaginoplasty. The molecular mechanism which hUSC-Exos-piGEL promotes healing by regulating the PI3K/Akt signaling pathway will be further elucidated.