创伤性脑损伤是一个非常严重的社会医学问题，脑损伤后神经功能修复是临床救治的难题，如何诱导内源性神经干细胞进行中枢神经系统修复已成为脑损伤治疗的新兴热点，直接关系到患者的预后与生存质量。本研究拟采用国际公认的Feeney法（自由落体冲击法）制备脑损伤大鼠模型，进行针刺治疗，采用透射电镜、 免疫荧光双标、液态蛋白芯片、荧光定量PCR等方法观察针刺对脑损伤模型大鼠神经干细胞增殖、分化及其正负调控基因Notch1、Hes1、Hes5、Sox-2、Wnt-3a的mRNA及蛋白表达的影响。从针刺调控多种相关基因表达、诱导内源性神经再生的角度探讨其治疗脑损伤的可能作用机理，为针刺修复受损中枢神经、治疗颅脑损伤提供可靠的实验依据；同时也将为针刺治疗中枢神经系统疾病提供新的思路和研究方向。

Traumatic brain injury (TBI) is a serious social medical problem. It is a challeng to repair nervous function after brain injury. How to induce endogenetic neural stem cells to repair central nervous system has already been a hot point, which is related to the prognosis and survival quality of the patients. In the present study, the adopted Feeney method and acupuncture were used to make and treat the rat model of TBI respectively. By using electronic microscopy, immunohistochemistry, liquid protein CMOS chip, fluorescence quantify PCR, etc, the effects of acupuncture on the proliferation and differentiation of endogenetic neural stem cells, the mRNA and protein expressions of the related gene Notch1, Hes1, Hes5, Sox-2 and Wnt-3a were observed, in order to study acupuncture mechanisms for TBI from the points of regulating the related gene expressions and inducing endogenetic neural regeneration.This study will not only consequently provide with experiential basis for the acupuncture repair of central nervous system and treatment of TBI,but also supply a new study strategy and direction for the acupuncture treatment of central nervous system.