发展微型化、便携式传感平台用于快速、精准、多元化的床旁检测，对疾病诊断和监控均具有重要的应用价值。本项目拟结合反向电渗析技术、双极电极微加工技术、新型纳米材料增敏方法，开发便携式盐差能驱动电化学发光传感平台、发展床旁检测新方法。将具有离子选择性、大面积且价格低廉的有机/无机复合介孔二氧化硅膜材料串联构筑浓差能源转换电源。利用纳米材料构筑导电性能好、抗干扰能力强、生物相容性好的双极电极电化学发光传感界面。借助微加工技术构筑双极电极阵列检测系统，通过同时记录所有阵列的电化学发光成像，建立血清中三种卵巢癌肿瘤标志物多组分同时检测的方法。本项目旨在发展实用、便携式、微型化的盐差能驱动双极电极电化学发光传感平台，以血清中卵巢癌肿瘤标志物为分析模型，推动卵巢癌早期诊断技术向便携化、微型化、多组分同时检测方向发展。

Development of a miniaturized and portable sensing platform for rapid, accurate and multiplexed point-of-care testing (POCT) is vital for disease diagnosis and monitoring. The project aims to develop a portable electrochemiluminescence (ECL) sensing platform powered by salinity gradient energy, and to develop a new strategy for POCT, combining reverse electrodialysis technique, bipolar electrode micro processing technique and signal amplification based on nano materials. Patches of organic/inorganic hybrid mesoporous silica membranes with ion-selectivity, large area and low cost can be used as salinity-gradient-driven power source to drive electrochemical reaction on bipolar electrode. Nano materials can be used to construct the ECL sensing interface on bipolar electrode with high conductivity, good anti-interference ability and biological compatibility. Multiplexed bipolar electrode arrays prepared by using micro processing technique can be used to simultaneously detect 3 tumour biomarkers of ovarian cancer in human serum by recording ECL images of all arrays. This project provides a practical, portable, miniaturized ECL sensing platform on bipolar electrode powered by salinity gradient energy, and 3 tumour biomarkers of ovarian cancer as models, which could make the early diagnosis of ovarian cancer more portable, miniature and multi-functional.