开花是林木生长发育过程中的一个重要阶段，研究林木的花发育调控能够有效改善林木的生长周期。本项目以前期获得的BpAP1转基因白桦为试材，首先采用染色质免疫共沉淀和烟草瞬时转化验证BpAP1与BpAP3之间的调控关系，同时进行白桦的遗传转化，对BpAP3基因的功能进行研究。采用RNA-seq鉴定差异表达基因，采用ChIP-seq技术找到BpAP3的直接靶基因，整合RNA-seq和ChIP-seq的结果获得BpAP3所调控的直接下游靶基因，从而构建BpAP3调控的基因网络，最终揭示BpAP1-BpAP3调控通路在白桦花发育过程中的分子机理，为丰富林木的花发育调控机制提供一定的参考及理论依据。

Flowering is an important stage in the process of forests growth and development. Research flower development regulation can effectively improve the growth cycle of forests. In this project, BpAP1 transgenic birch were used as materials. Chromatin immunoprecipitation and tobacco instantaneous transformation experiments were used to verify the regulation relationship between BpAP1 and BpAP3. The function of BpAP3 gene were studied. RNA-seq was employed to identify the differentially expressed genes, and then we hope to screen the binding site of BpAP3 within the scope of genome-wide by employing ChIP-seq. RNA-seq and ChIP-seq were integrated to determine the target genes that directly regulated by BpAP3. Finally, we analyze the relationship between BpAP3 and other genes and build the BpAP3 transcription regulatory network. Our results would reveal molecular mechanism of BpAP1-BpAP3 regulation pathway in flower development of birch, and provide an important reference and theoretical basis for the folwering development regulatory mechanism of forests.