转录因子U-shaped和Lozenge对家蚕血细胞发生与免疫调控机理研究

本项目针对家蚕存在抗逆性差，对病害的免疫低和抵抗力弱的问题，以阐明家蚕关键转录调控因子在血细胞增殖分化和家蚕先天性免疫反应中的作用及分子机理等基本科学问题为目标，拟开展家蚕血细胞特异性启动子鉴定，构建血细胞特异性基因编辑体系，鉴定U-shaped和Lozenge相互作用，阐述其在血细胞增殖分化和免疫防御反应中的调控作用和分子机制，解析U-shaped和GATA-6复合体在细胞程序性死亡中的调控作用及其下游靶基因。通过RNAi、CRISPR/Cas9基因编辑和转基因等技术，进行家蚕血细胞关键调控因子的创新研究，提出家蚕血细胞增殖分化和免疫反应的调控理论。通过本项目的研究，能达到丰富家蚕血细胞增殖分化和免疫调节网络，改善和增强家蚕自身免疫防御能力，加速抗性品系培育的目的，同时也可以为农林病害防治提供理论支撑，对我国的蚕桑丝绸等农业领域的发展具有重大意义。

In order to cope with issues such as poor stress resistance, low immunity, and weak resistance of silkworms and target at illustrating function, molecular mechanism and other basic scientific issues of key regulatory factors of silkworm hemocytes in proliferation and differentiation and immunity. We propose to 1) identificate hemocytes-specific promoters; 2) establish hemocyte-targeted gene editing system; 3) identificate the interactions of U-shaped and Lozenge, and their regulation roles in hemocytes proliferation and differentiation; 4) elucidate the regulation of U-shaped and GATA-6 compounds on programmed death of cells and analysizing of their downstream target genes. Technologies such as RNAi and CRISPR/Cas9 gene editing as well as transgenic technology are adopted for innovative study of this project to put forward elaborated regulation theory of proliferation, differentiation and immunoreaction of silkworm hemocytes. This project can focuses on proliferation, and differentiation and immunoregulation network of silkworm hemocytes, improvement and enhancement in autoimmune defense of silkworms. These discoveries provide theory support for agriculture disease control and hold potential in further development into significant technical guidance in sericulture and silk industry in China.