等离子体可高效失活伤口表面定植的金黄色葡萄球菌（S.aureus）生物膜，但膜中残留的少量存活细菌具有再次生成生物膜从而诱发伤口二次感染的生物安全性隐患。等离子体中活性含氧/氮基团（RONS）被认为是影响S.aureus生物膜再生特性的关键因子，然而相关作用机制尚不明晰。. 本项目拟先通过生物学表征手段系统评价：针对性提高等离子体中不同种类气相RONS（O、OH或NOx）的强度/占比后，S.aureus生物膜再生特性的变化规律；随后利用等离子体诊断技术和化学分析方法，揭示上述等离子体中气相RONS及其引发浸润生物膜的血清中生成的液相RONS的种类/强度（含量）变化和调控规律；在此基础上综合考察液相RONS与S.aureus生物膜再生特性之间的生物响应关系，并引入专一性RONS清除剂加以验证，最终阐明作用机制。研究结果可为等离子体治疗伤口生物膜感染的临床应用提供实验数据支持。

Plasma could inactivate the Staphylococcus aureus (S. aureus) biofilms located on the wounds’ surfaces with high efficiency. However, the remaining few survival bacteria encased in the plasma-treated biofilms possess the biosafety risks in regenerating biofilms thus triggering wound re-infections. Plasma-generated reactive oxygen/nitrogen species (RONS) are considered as key factors in affecting the biofilm regeneration characteristics of S. aureus. However, the specific mechanisms are still unclear. In this project, multiple biological analytical methods are adopted to systematically evaluate the change rules on the S. aureus biofilm regeneration characteristics triggered by plasmas which contain specific types of gaseous RONS (O, OH or NOx) with targetedly increasing intensity/proportion, respectively. Afterwards, the plasma diagnostic techniques combined with various chemical detection methods are utilized to reveal the variation and regulation rules on the type/intensity (content) of the gaseous RONS in abovementioned plasmas and the plasma-induced aqueous RONS in the blood serum which soaks microbial biofilms. On this basis, the biological responses are comprehensively investigated between the aqueous RONS and the S. aureus biofilm regeneration characteristics. Moreover, multiple specific scavengers for particular aqueous RONS are introduced into the research system to verify the obtained biological responses, thus finally illuminating the related mechanisms on the S. aureus biofilm regeneration characteristics by the plasma-generated RONS. The research results can provide experimental data support for the clinical application of the plasma therapy on the biofilm-driven wound infections.