迁移体是近年被发现并命名的新型细胞器。在细胞迁移过程中，细胞后端的收缩丝上会逐渐形成直径约1-3微米且包含多种信号分子的囊泡状结构，即为迁移体。伴随着内容物的释放或被其他细胞吞噬，迁移体在细胞间信息物质交流方面发挥着重要作用。在迁移体的形成过程中，四次跨膜蛋白质Tetraspanin4局部富集形成微结构域并进一步组织成为微米级巨结构域，从而增加局部膜的弯曲刚度和形变，驱动迁移体的形成。然而，目前Tetraspanin4富集的微结构域及巨结构域的形成机制仍没有被揭示。因此，本项目拟通过建立体内及体外研究系统，综合细胞生物学及生物物理学的研究思路和方法，对Tetraspanin4富集的微结构域及巨结构域的理化性质、形成机制及其形成过程的调控机制展开逐层深入的研究，旨在从物理层面进一步解释迁移体形成的分子机制。

Migrasome is a newly discovered organelle which forms on the tip of retraction fibers of migrating cells. Migrasome play important roles in cell-cell communication by releasing signaling molecules. Migrasome formation is mediated by assembly of Tetraspanin4 enriched microdomain to micron scale macrodomains. How Tetraspanin4 enriched microdomains are formed and how microdomains assemble into macrodomains are still unknown. In this proposal, we propose to investigate 1) the chemical composition and mechanic property of Tetraspanin4 enriched micro/macro domain, 2) the mechanisms for formation and regulation of Tetraspanin4 enriched micro- and macro- domains. By combining cell biology and biophysics and modeling approach, we aim to understand the formation of migrasome in molecular and physical term.