基于拉伸作用诱导血管内皮细胞炎症反应的血管早期病变过程研究

本项目以构建类似血管弯曲部位的非均一性拉伸环境和平直区域的均一性拉伸环境为出发点，诱导血管内皮细胞的炎症反应，分析炎症反应相关细胞因子的相互作用。通过建立血管内皮细胞、平滑肌细胞和成纤维细胞共培养体系，引入相关免疫细胞（单核细胞、中性粒细胞、淋巴细胞），模拟人体血管生理环境，诱导血管病变的早期关键事件：包括动脉粥样硬化中的泡沫细胞形成与动脉瘤中的平滑肌细胞增生与凋亡。探讨血管病变发生过程的关键因素，为血管疾病的防治提供重要依据。

In this project, constructing two different strain profiles to simulate heterogeneous and homogenous cell stretching behaviors at bending and straight blood vessel, respectively, acts as a starting point. Based on the stretch-induced inflammatory reaction of vascular endothelial cells, the variation of inflammation related cytokines will be investigated. With the establishment of vascular endothelial cells, smooth muscle cells and fibroblasts in co-culture system and the introduction of related immune cells (monocytes, neutrophils, and lymphocytes), the physiological environment of blood vessel is to be simulated, in which the early critical events of vascular lesions are planned to take place, such as foam cell formation (atherosclerosis) and SMC proliferation and apoptosis (aneurysm). It is anticipated that our investigation on the key factors of vascular diseases process can provide important basis for prevention and treatment of vascular diseases.