人工肺泡环境下大气可吸入颗粒物与细胞之间相互作用研究

本项目基于微流控芯片技术构建人工肺泡模型，考察大气可吸入颗粒物与肺泡细胞之间的相互作用，探讨颗粒物引起的炎症反应和上皮细胞间充质转化机制。研究内容包括制备具有伸缩作用、气-液界面、气-血屏障等生理特征的人工肺泡环境；活细胞成像观测颗粒物穿越由肺上皮细胞和血管内皮细胞共培养形成的气血屏障；揭示颗粒物引起的炎症反应和上皮间充质转化分子机制；提供大气可吸入颗粒物损害肺泡的科学依据，以加强治理大气污染的决心。

This project is based on Microfluidic Chip technology to build an artificial alveolar model for investigation of the interactions between the alveolar cells and atmospheric inhalable particles, study the mechanisms of particle induced epithelial cell inflammation and epithelial mesenchymal transformation. The research mainly includes to build an artificial alveolar environment with some physiological features, such as strechable, gas-liquid interface, and gas-blood barrier; live cell Imaging observation of the particles passing through a gas-blood barrier formed by lung epithelial cell and vascular endothelial cell; discover the relationship among the particles, inflammation reaction, and epithelial mesenchymal transformation; providing the evidences of atmospheric inhalation particles caused damage to alveolar cell, to strengthen the governance determination to control atmosphere pollution.