体外流体环境下内皮和平滑肌细胞共培养与细胞行为的研究

本项目利用一种凹槽型微流控可拉伸芯片在发生形变后产生的管状结构，将血管内皮细胞和平滑肌细胞共培养于管状结构的内壁。在管内提供持续流动的细胞培养基模拟血液流动的特征，产生作用于管壁细胞的剪切力，环向拉伸、液体压力效应。在此基础上，考察不同程度的剪切力和环向拉伸对一氧化氮合酶（eNOS）功能、细胞氧化应激反应、NADPH氧化酶表达、平滑肌细胞表型变化、增殖、凋亡，以及胞外基质蛋白等等细胞行为的影响；考察薄膜弹性对内皮细胞和平滑肌细胞上述行为的影响以及细胞外基质蛋白的表达变化；考察流体环境下的循环体系因素（葡萄糖浓度、低密度脂蛋白浓度、激素等）对eNOS功能、细胞炎症反应、细胞内脂滴积累的影响。通过上述研究为治疗血管疾病和相关治疗药物的筛选方法提供科学依据。

This project uses a grooved microfluidic stretchable chip to produce a tubular structure after deformation, and co-cultures vascular endothelial cells and smooth muscle cells on the inner wall of the tubular structure. The continuous flow of cell culture medium is provided in the tube to simulate the characteristics of blood flow, which produces shear force , circular stretching, and liquid pressure effects on the cells of the tube wall. On this basis, we are going to investigate the effects of different degrees of shear and loop stretching on the function of nitric oxide synthase (eNOS), cellular oxidative stress, NADPH oxidase expression, smooth muscle cell phenotypic changes, proliferation, and apoptosis, as well as the influence of extracellular matrix proteins and other cell behaviors; investigate the influence of film elasticity on the above-mentioned behaviors of endothelial cells and smooth muscle cells and the expression changes of extracellular matrix proteins; investigate the circulatory system factors (glucose, low-density lipoprotein, hormones, etc.) in a fluid environment acting on eNOS function, cellular inflammatory response, intracellular lipid droplet accumulation; The above-mentioned researches are anticipate to provide scientific basis for the treatment of vascular diseases and the screening method for related drugs.