多糖及糖复合物是生命体的重要组成部分，在创面再生修复过程中起着重要作用。我们前期受大鲵自我保护机制启发，利用大鲵皮肤分泌粘液提高创面愈合速率和质量，发现粘液中的糖胺聚糖可显著提高新生血管和成熟血管相关标记物CD31和α-SMA表达，显示出促血管生成效能，但其结构和促修复机制尚不明确。本项目拟通过收集大鲵体表粘液中不同分子量的糖胺聚糖（SSAD-GAGs），基于FT-IR光谱、GC-MS、NMR等技术手段对其进行结构表征，利用表面等离子体共振技术检测SSAD-GAGs与血管生成相关生长因子的高度亲和性，揭示多糖分子量大小和糖醛酸含量等因素对促进血管生成过程的影响规律，阐明SSAD-GAGs增强创面血管化的功能结构和作用机制；结合安全性毒理学初步评价，发现更加高效安全且适用于临床的增强和解决患者伤口愈合过程的糖胺聚糖先导物质指导下游创面敷料的设计与制备，为未来的临床治疗带来新途径和新方法。

As an essential part of life entity, polysaccharides and their complexes play an important role in wound healing. Inspired by the self-protective mechanisms of giant salamanders, in the early stage, we have confirmed skin secretion of Andrias davidianus (SSAD) significantly improved the rate and quality of wound healing. Further experiments showed that glycosaminoglycan in the mucus could significantly improve the expression of CD31 and α-SMA, which is an important manifestation of its angiogenic property. However, the structures and mechanism pro-angiogenesis of SSAD-GAGs are still unclear. This project intends to collect glycosaminoglycans of different molecular weights by means of size exclusion chromatography, and characterize their structures by Raman spectroscopy, FTIR spectroscopy, LC-MS, NMR. And, detect the high affinity between SSAD-GAGs and angiogenesis related growth factors by using surface plasmon resonance technique (SPR). Meanwhile, obtain a more effective and safe glycosaminoglycan as a lead material by combined with the preliminary evaluation of biocompatibility, to enhance and solve the wound healing process of patients, and guide the design and preparation wound dressings in the future. Therefore, it would bring new approaches and methods for future clinical treatment.