SPLUNC1是一种在鼻咽组织特异性表达的具有免疫作用的分泌蛋白，而在鼻咽癌组织中表达明显下调。前期研究发现SPLUNC1能够抑制EB病毒DNA诱导的炎症因子IL-6，IL-8的分泌，将转染EB病毒DNA的巨噬细胞培养上清处理鼻咽癌细胞系，发现SPLUNC1能够抑制细胞上清引起的鼻咽癌细胞的增殖和细胞周期进程，但其分子机制以及SPLUNC1抑制EB病毒DNA所诱导的炎症反应是否参与抑制鼻咽癌的发生发展有待进一步研究。为此，本课题将系统的研究SPLUNC1抑制EB病毒DNA诱导的炎症反应的分子机制及其是否通过抑制EB病毒DNA诱导炎症反应进而抑制鼻咽癌细胞的恶性表型。并利用大样本分析SPLUNC1、IL-6以及EBV-VCA-IgM联合作为鼻咽癌早期诊断分子标志物的价值。本研究首次从SPLUNC1抗EB病毒诱导炎症的角度为SPLUNC1参与鼻咽癌发生发展提供新机制。

SPLUNC1 is a secreted innate immune molecule which is tissue-specific expressed in nasopharyngeal epithelia, but was down-regulated obviously in nasopharyngeal carcinoma. Our previous studies showed that SPLUNC1 could suppress the expression of inflammatory cytokines（IL-6,IL-8）induced by EB virus DNA ; using culture supernatant of macrophage transfected with DNA of EB virus to treat nasopharyngeal carcinoma(NPC) cell line, we found that SPLUNC1 could inhibit the proliferation and arrest cell cycles of NPC cell line, however, its molecular mechanism need be further studied. This project will investigate whether SPLUNC1 can inhibit the malignant phenotype of NPC cells through interfering the inflammatory response induced by EB virus DNA, and using a large of samples to analysis the value of SPLUNC1,IL-6 and EBV-VCA-IgM as an early diagnosis molecular marker of NPC. Our studies will provide a new insight to understand the mechanism of SPLUNC1 involved in the initiation and development of nasopharyngeal carcinoma.