株型是指植物形态特征及各器官的空间排列方式，决定着植株受光姿态，直接影响作物的光合效率。株型相关基因及调控机制的研究对作物高效生产具有重要的理论和实践意义。黄瓜株型相关分子机制研究较少，申请者前期通过诱变得到黄瓜微株型突变体‘mp’，植株节间缩短且各器官表现微型化，利用Mutmap方法定位并克隆获得微株型关键候选基因CsKTNp60，该基因可编码微管剪切蛋白Katanin p60，突变体仅一个碱基变异，但该基因调控黄瓜株型的分子机制仍未知。本项目拟通过表达分析、遗传转化及TILLING等技术验证候选基因CsKTNp60，解析该基因的株型调控功能；同时通过体内/体外微管结合实验、转录组分析等方法明确微管剪切蛋白在黄瓜细胞形态建成及株型调控中的生物学功能。研究结果不仅可以丰富黄瓜株型形成的理论知识，为培育黄瓜理想株型新品种提供理论依据，同时也为研究植物微管剪切蛋白的代谢及调控机制提供重要参考。

Plant type refers to the morphological characteristics and spatial arrangement of the plant and organs, which determines the photosynthetic posture of the plant and directly affects the photosynthetic efficiency of the crop. Researches about the genes related to plant type and their function play important roles in the high-efficient production of crops. Researches about molecular mechanism of cucumber plant type are very few. By chemical mutation method in our previous researches, we obtained a micro-plant type cucumber mutant ‘mp’, which has significantly shortened internode and miniaturized organs. We used Mutmap method to map and identify a candidate gene, CsKTNp60, which encodes the microtubule cleavage protein Katanin p60. Only one nucleotide base mutation was found between wild type and mp. However, the molecular mechanism of this gene regulating the cucumber plant type is still unknown. In this project, we plan to carry out the analysis of gene expression, genetic transformation and TILLING technology to verify the candidate gene CsKTNp60, clarifying its regulation mechanism for micro-plant type of cucumber. Further, through in vivo / in vitro microtubule binding experiments and RNA-seq analysis, we will investigate deeply the biological function of microtubule cleavage protein in cucumber cell and plant architecture formation. The expected results from this project will not only enrich the theoretical knowledge of cucumber plant architecture formation and provide reliable theoretical basis for the ideal plant type breeding of cucumber in future, but also provides an important reference for the study of the metabolism and regulation mechanism of plant microtubule cleavage protein.