乙型肝炎病毒（HBV）感染是肝细胞肝癌发生的主要诱因。主要穹窿蛋白（MVP）是穹窿蛋白复合物的一个组成部分，它在调节细胞耐药性、细胞应激反应、细胞自噬等方面起到重要作用。我们前期研究发现，HBV感染诱导MVP表达。免疫沉淀结合质谱分析发现MVP与癌基因鼠双微体2（Mdm2）相互作用。结果提示，MVP在HBV相关性肝癌发生发展中起重要作用。目前尚无研究报道MVP与HBV相关性肝癌发生发展中之间的关系。我们在前期工作的基础上提出假说：HBV诱导MVP结合Mdm2，进而调控p53的功能，最终导致了肝癌发生发展。据此，本项目有以下两部分内容：第一部分是利用临床样本、小鼠模型和细胞模型研究MVP在HBV相关性肝癌发生发展中的作用。第二部分是研究MVP调控HBV相关性肝癌发生发展的机制。这些研究将为抗肝癌药物的研发提供新信息和策略。

Hepatitis B virus (HBV) infection is the main cause of hepatocellular carcinoma (HCC). Major vault protein (MVP) is the major constituent of vaults and is involved in multidrug resistance，stress responses and autophagy. Our previous research found that HBV infection induced MVP expression. Co-IP-MS assays demonstrate that MVP associates with murine double minute 2 (Mdm2). Those results indicate that MVP play an important role in HCC. However, little is known about the role of MVP in HBV-related hepatocellular carcinoma. Based on previous work, we propose the following hypotheses: During the viral infection, MVP associates with Mdm2 to inhibit p53 function, lead to the development of HCC. According to this model, we plan to study the following two parts: The first part is to find the role of MVP in HBV-related hepatocellular carcinoma by using clinical samples, mouse model and cell model. The second part is to study the regulatory mechanism of MVP in HBV-related hepatocellular carcinoma. This project will provide new information and strategies for the development of anti-hepatocellular carcinoma drug candidates.