肾损害是类风湿关节炎（RA）主要并发症之一，因其起病隐匿且特异性症状少，往往在出现肾功能不全乃至尿毒症时才被确诊，延误治疗、影响预后。因此早期诊断以实现早期治疗至关重要。课题组前期基于代谢组学技术建立了多种疾病的早期生物标志物筛选方法。本研究针对RA病程演变过程中的肾损害现象，利用“代谢组学技术结合支持向量机等方法”对不同阶段RA肾损害大鼠的生物样本进行动态分析，结合生化、病理改变分析肾功能及病理形态变化，筛选并确认RA肾损害的早期预警标志物，同时构建其检测方法；进而将其应用于中药防治RA肾损害的模型中以验证所筛选的早期预警标志物，鉴于雷公藤与白芍防治RA的过程中分别具有诱发和抑制肾损害的作用，拟选用胶原诱导性关节炎大鼠模型，从正反两方面进一步验证及评价RA肾损害早期预警标志物的适用性。本研究将为RA肾损害的早期诊断提供新的思路和方法，同时为RA的精准治疗及其治疗策略提供方法学依据。

Renal damage is one of the major complications of rheumatoid arthritis. Because of its insidious occult onset and specific symptoms, it can be diagnosed often waiting until there is renal insufficiency and uremia. This will delay treatment and affect prognosis. Therefore, early diagnosis to achieve early treatment is crucial.. Our research group established the early biomarker screening method for various diseases based on the metabonomics. Aiming at the phenomenon of renal demage during the course of RA, this study going to analysis the biological samples of rats with RA renal damage in different stages by “metabolomics techniques combined with support vector machines and other methods”, analysis of renal function and pathological changes combined with the biochemistry and pathological changes, it will eventually screen and confirm the early warning biomarkers of RA renal damage, and to build its detection method. And then apply it in the model of prevention and treatment of RA renal damage by Chinese medicine to verify the selected early warning markers. Tripterygium can treat RA but can cause kidney damage, Radix Paeoniae can treat RA and inhibit renal injury. Based on this, this study intended to use collagen induced arthritis rat model, and to further validate and evaluate the applicability of early warning markers of RA renal damage in both positive and negative aspects. This study will provide new ideas and methods for the early diagnosis of renal damage in RA, and provide an important basis for the precise treatment of RA and its treatment strategy.