碳水化合物的种类和结构十分复杂，对猪健康和机体代谢影响差异甚大，确保猪最佳健康和生长的碳水化合物营养平衡模式未见报道。碳水化合物的不同效应与肠道微生物及其与宿主的互作有关，然而肠道微生物介导不同碳水化合物营养模式的机制尚不清楚。本项目拟比较研究不同种类和组合的碳水化合物（淀粉、NSP及寡聚糖）对猪肠道微生物菌群结构及肠道屏障与糖脂代谢影响，弄清碳水化合物、肠道微生物与宿主的关系；通过无菌猪模型与菌群移植阐明肠道微生物在日粮碳水化合物—宿主互作中的介导作用；通过微生物代谢产物灌服及相关信号通路分析初步揭示肠道微生物与宿主代谢的对话机制。通过研究，旨在建立仔猪碳水化合物营养平衡模式，初步探明肠道微生物的介导机制。研究结果对于深入认识碳水化合物—肠道微生物—宿主的互作机制、丰富碳水化合物营养原理、创建碳水化合物营养平衡技术具有理论与实践意义。

The types and structures of carbohydrates are very complicated, and have various effects on pig health and body metabolism. However, the optimal balance pattern of different carbohydrates for pigs is still unknown. The effects of carbohydrates must be closely related to gut microbiota and its interactions with host, its mechanism, however, is not very clear. The present project aims to understand the relationship among carbohydrates, gut microbiota and host response by comparing the effects of different types(starch, NSP and oligosaccharides) and patterns of carbohydrates on gut microbiota composition, gut barrier function and glyco-lipid metabolism, to clarify the function of gut microbiota in mediating interactions between carbohydrates and host metabolism by utilizing germ-free pig model and transplanting fecal microbiota, and to reveal the possible crosstalk mechanisms of gut microbiota and host metabolism by infusing SCFAs and detecting the expressions of passway-related target proteins. After completion of the above series of studies, the optimal dietary carbohydrate balance pattern for piglets will be established and possible mediating mechanism of gut microbiota will be elucidated. This study will be of both theoretical and practical value for further understanding interactions among carbohydrates, microbiota and host, for enriching the principles of carbohydrate nutrition, and for developing technology of carbohydrate balance for pigs.