Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota.
Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota.
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短链脂肪酸可以独立于猪肠道微生物群改善脂质和葡萄糖代谢
DOI：
10.1186/s40104-021-00581-3
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发表时间：
2021-05-06
影响因子：
7
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Zhou H;Yu B;Sun J;Liu Z;Chen H;Ge L;Chen D
以往的研究表明，外源性短链脂肪酸（SCFAs）的引入减弱了传统小鼠和猪的体脂沉积。然而，有限的研究已经评估了外源引入的SCFA对脂质和葡萄糖代谢的影响，而不依赖于肠道微生物群。本研究旨在探讨外源性SCFAs对无菌猪脂肪代谢和葡萄糖代谢的影响。12头切除盲肠的新生猪在6个无菌隔离器中饲养。用无菌奶粉人工饲喂21 d后，再用无菌饲料喂猪21 d。在第二个21 d阶段，每天灌胃25 mL/kg无菌生理盐水，作为GF组;每天灌胃25 mL/kg SCFAs混合物（乙酸、丙酸和丁酸，分别为45、15和11 mmol/L），作为FA组。口服SCFAs可提高血清脂联素水平，增强背最长肌CPT-1活性，上调结肠ANGPTL 4 mRNA表达水平（P < 0.10）。FA组肝脏ACC、FAS、SREBP-1C和背最长肌CD 36的mRNA丰度均低于GF组（P < 0.05）。FA组肝脏PGC-1α和背最长肌LPL mRNA表达分别呈上调和下调趋势（P < 0.10）。此外，口服SCFAs有升高肝脏GPR 43蛋白水平（P < 0.10）和降低ACC蛋白水平（P < 0.10）的趋势。同时，SCFAs可上调肝脏中p-AMPK/AMPK比值及GLUT-2和GYS 2的mRNA表达（P < 0.05）。此外，与不饱和脂肪酸的生物合成相关的代谢途径通过口服SCFAs被最显著地促进（P < 0.05）。外源性引入SCFA可能会减弱脂肪沉积，并在一定程度上改善猪模型中的血糖控制，这与肠道微生物群无关。在线版本包含补充材料，可通过10.1186/s40104-021-00581-3获得。
Previous studies have shown that exogenous short-chain fatty acids (SCFAs) introduction attenuated the body fat deposition in conventional mice and pigs. However, limited studies have evaluated the effects of exogenously introduced SCFAs on the lipid and glucose metabolism independently of the gut microbiota. This study was to investigate the effects of exogenous introduction of SCFAs on the lipid and glucose metabolism in a germ-free (GF) pig model. Twelve hysterectomy-derived newborn pigs were reared in six sterile isolators. All pigs were hand-fed with sterile milk powder for 21 d, then the sterile feed was introduced to pigs for another 21 d. In the second 21-d period, six pigs were orally administrated with 25 mL/kg sterile saline per day and considered as the GF group, while the other six pigs were orally administrated with 25 mL/kg SCFAs mixture (acetic, propionic, and butyric acids, 45, 15, and 11 mmol/L, respectively) per day and regarded as FA group. Orally administrated with SCFAs tended to increase the adiponectin concentration in serum, enhance the CPT-1 activity in longissimus dorsi, and upregulate the ANGPTL4 mRNA expression level in colon (P < 0.10). Meanwhile, the mRNA abundances of ACC, FAS, and SREBP-1C in liver and CD36 in longissimus dorsi of the FA group were decreased (P < 0.05) compared with those in the GF group. Besides, the mRNA expression of PGC-1α in liver and LPL in longissimus dorsi tended to (P < 0.10) upregulate and downregulate respectively in the FA group. Moreover, oral administration of SCFAs tended to increase the protein level of GPR43 (P < 0.10) and decrease the protein level of ACC (P < 0.10) in liver. Also, oral administration of SCFAs upregulated the p-AMPK/AMPK ratio and the mRNA expressions of GLUT-2 and GYS2 in liver (P < 0.05). In addition, the metabolic pathway associated with the biosynthesis of unsaturated fatty acids was most significantly promoted (P < 0.05) by oral administration of SCFAs. Exogenous introduction of SCFAs might attenuate the fat deposition and to some extent improve the glucose control in the pig model, which occurred independently of the gut microbiota. The online version contains supplementary material available at 10.1186/s40104-021-00581-3.
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影响因子：
7.7
作者：
den Besten, Gijs;Bleeker, Aycha;Bakker, Barbara M.
通讯作者：
Bakker, Barbara M.
影响因子：
4.1
作者：
Balampanis, Konstantinos;Chasapi, Athina;Sotiropoulou-Bonikou, Georgia
通讯作者：
Sotiropoulou-Bonikou, Georgia
影响因子：
4.2
作者：
HOVERSTAD, T;MIDTVEDT, T
通讯作者：
MIDTVEDT, T
影响因子：
29
作者：
De Vadder, Filipe;Kovatcheva-Datchary, Petia;Mithieux, Gilles
通讯作者：
Mithieux, Gilles
影响因子：
3.5
作者：
Carbone, Salvatore;Mauro, Adolfo G.;Toldo, Stefano
通讯作者：
Toldo, Stefano