巨噬细胞作为结核分枝杆菌的靶细胞，它与结核分枝杆菌相互作用后发生的凋亡与坏死对结核病的发生、发展及预后具有重要影响，因此，阐明 该过程的调控机制具有十分重要的科学意义。近年研究表明，LncRNA在多种疾病的发生发展过程中发挥了重要的调控作用，但是其在结核分枝杆菌与巨噬细胞的相互作用过程中对细胞凋亡与坏死的调控作用尚未见相关报道。. 基于以上研究背景，本项目拟采用LncPath™ 疾病/信号通路特异性LncRNA芯片筛选结核分枝杆菌感染巨噬细胞后与宿主细胞凋亡/坏死信号通路相关的LncRNA分子，通过构建其过表达载体和干扰载体并转染RAW264.7细胞后并用结核分枝杆菌感染，进而用caspase抑制剂和RNAi技术分别抑制和沉默凋亡与坏死通路相关基因，以期阐明LncRNA对巨噬细胞凋亡和坏死的调控作用及其分子机制。研究结果将为深入理解结核病的发病机制及靶向药物研制提供理论依据。

As target cell of mycobacterium tuberculosis, Apoptosis and necrosis of Macrophages after infection with Mtb play an important role in the development and prognosis of the disease, therefore, to clarify the regulatory mechanism of the process has important scientific significance. In recent years, studies have shown that LncRNAs are involved in the development of a variety of disease, but the regulatory role of LncRNAs in apoptosis and necrosis of Macrophages after infection with Mtb remains unclear.. Based on the research background, the present study aims to identify LncRNAs related to apoptosis and necrosis of macrophage infection with Mtb through LncPath ™ disease/signaling pathways microarray. In order to clarify regulatory role of LncRNAs in apoptosis and necrosis of Macrophages infection with Mtb. overexpression vector and siRNA vector will be constructed to transfect RAW264.7 cells respectively, and then the transfected macrophage will be infected with Mtb, Furthermore, the caspase inhibitor and RNAi technology will be used to inhibit and silence apoptosis and necrosis pathway genes respectively. The research results will provide further insight into the pathogenesis of tuberculosis (TB) and theory basis for development of targeted drugs.