脑动静脉畸形(bAVMs)是脑内动静脉直接沟通的异常血管团，畸形团长期受高血流压力的冲击，易导致破裂出血。本课题旨在探讨脑动静脉畸形发生发展机制过程中的血管生成和重塑。酪氨酸激酶家族配体EphrinB2是循环系统发育过程中最早出现的动脉血管基因标志物，影响血管生成和重塑，调控血管内皮细胞的分化和调节血管发育的口径。故假设，EphrinB2是脑动静脉畸形血管生成的关键调控因子，而血流动力学因素可能影响其表达。本课题拟根据术中B超检测血流阻力指数分组，对动静脉畸形病人术中切除畸形血管及对照组正常标本进行体内外实验，运用RT-PCR、WESTERN-BLOT、免疫组化以及活体细胞分离和三维立体培养等实验技术，评估EphrinB2在畸形血管中的表达差异，并进一步探讨血流动力学参数、EphrinB2、VEGF及Notch4基因表达之间的关系，了解脑动静脉畸形血管生成调控过程的关键途径。

Brain AVMs are defined as the direct communication of arteries to abnormally tortuous and dilated veins without interposing capillaries.Traditionally, they have been thought of as congenital lesions resulting from abnormal vascular development，However, many authors have suggested that they are not static congenital lesions, but are dynamic with the ability to grow, regress, and even reappear as de novo brain AVMs after complete resection or radiosurgery.Angiogenesis is defined by morphogenic changes hallmarked by ongoing "pruning" of the premature vascular plexus, the formation of new branches, and.stabilization of the definitive vessel.EphrinB2, a transmembrane ligand of EphB receptor tyrosine kinases, is specifically expressed in arteries,which promot angiogenesis.Dynamic in AVMs vivo models are a potential research area. The goal of this research is to identify the relationship between ephrinB2 and brain AVMs under the intraluminal pressure.The IHC, WESTERN-BLOT , RT-PCR and cell culture will be used for the detecting the relationship between EphrinB2,VEGF,Notch4,bAVMs and Hemodynamic change.The identification of molecular targets is critical for targeted drug treatment.