紫菜是我国重要的经济栽培海藻，病害频发是限制产业发展的重大问题。黄斑病是条斑紫菜和坛紫菜丝状体育苗阶段常见的重大疾病，病因复杂，传染性强，会对紫菜栽培造成根本性损失。基于“环境-病原菌-藻体”三者相互作用的综合考量，本项目通过观察在温度、pH、盐度、拮抗菌、藻类激发子等多重环境因子作用下，病原菌（弧菌117-T6）种群数量和代谢物的变化，探究环境对病原菌感染紫菜丝状体能力的影响，明确黄斑病的致病原因与机理；通过观察紫菜在不同环境条件和病原菌共同作用下的生理与病理反应，结合转录组和代谢组数据，明晰紫菜黄斑病的病理特点，探寻紫菜丝状体在黄斑病发病过程中的标志性代谢物质及其相关代谢网络基因表达的变化规律。本研究旨在系统阐述紫菜丝状体黄斑病的致病机理及环境因子在其中的作用，为该病的防治提供参考。

Pyropia species were the most important cultivated seaweeds in China, the industries of which were greatly limited by the frequent diseases. Yellow-spot disease was one of the serious diseases occurred during the conchocelis breeding period of Pyropia yezoensis and Pyropia haitanensis. Because yellow-spot disease with strong infectiousness outbreaks at the very beginning of cultivation process and the nosogenesis is complicated, which might make radical damage to Pyropia cultivation. The tri-angle theory of “environment-pathogen-plant” is the principle strategy to study plant pathology. Temperature, pH, salinity, antagonistic bacteria and algae elicitor were considered as the multiple environmental factors in this research. The changes of biomass, metabolits of the pathogenic bacteria (Vibiro 117-T6) under different environmental conditions were studied, and the ability of infect conchocelis were evaluated meanwhile in order to reveal the mechanism of yellow-spot disease pathogenesis in Pyropia species. The physiological and pathological straits of both the free conchocelis and shell-born conchocelis of P. yezoensis and P. haitanensis were detected with the composite actions of environmental factors and the pathogens. Both the metabolomics and transcriptome data of Pyropia conchocelis were applied to reveal the key materials and the related genetic networks involved in the process of yellow-spot disease of Pyropia，which could constructs a metabolic network for the disease. This research systematically elaborate a comprehensive understanding of pathogenesis of the yellow-spot disease in Pyropia and the role of environmental factors in the course of disease, which could direct the prevention and control of the disease.