主栽葡萄品种抗寒性差，研究和利用抗寒基因扩大葡萄栽培范围、提高葡萄品质是葡萄产业健康发展的重要科学问题。脱水素是一类抗逆蛋白，在植物抵御低温胁迫中发挥重要的作用。本项目以前期研究获得的燕山葡萄抗寒脱水素基因VyDHN1为研究对象，研究内容包括：（1）在蛋白质水平研究VyDHN1的分子伴侣活性、亚细胞定位、互作的蛋白质；（2）通过转化烟草研究VyDHN1基因的调控与反馈机制；（3）研究过量和RNA干扰表达VyDHN1对砧木葡萄抗寒功能的影响；（4）通过微嫁接技术研究转VyDHN1砧木对接穗欧洲葡萄抗寒性的影响并探讨VyDHN1在接穗中的转录、表达情况。通过上述研究，以期明确VyDHN1调控砧木葡萄抗寒的分子机制和在嫁接体系中的作用，为转基因砧木葡萄抗寒的生产应用提供理论依据。

The cultivated varieties from Vitis vinifera are susceptible to cold. So it is vital for researching and using the cold resistance gene to expand the cultural area and improve the quality in green development grape industry. Dehydrins belong to plant resistance relative proteins, which play an important role in plant resistance to low temperature stress. The cold-resistant dehydrin gene VyDHN1 from Vitis yeshanensis obtained in previous studies is adopted in the present research, the project of the research include: (1) to study the chaperone activity, subcellular localization and interacting protein of VyDHN1 at protein level; (2) to explore the expression patterns and feedback mechanism of VyDHN1 by transformed into tobacco; (3) to clarify the cold resistance of VyDHN1 by overexpressed or RNA interference expressed into the rootstocks grape; (4) to find out the influence of the transcription and translation of VyDHN1 in Vitis vinifera scion by micrografted into the transformed rootstocks. This study is expected to explain the mechanism of VyDHN1 regulated the cold stress resistance in rootstock grape and the influence of VyDHN1 in the grafting system. In doing so, it will contribute to improve hardy rootstock grape breeding and cultivation.