胰腺星状细胞上皮-间质转化介导慢性胰腺炎向胰腺癌的演变

慢性胰腺炎和胰腺癌是常见疾病，慢性胰腺炎可以演变为胰腺癌，其机制尚不清楚。胰腺星状细胞（PaSC）在慢性胰腺炎的发生发展中起着最关键的作用，而最近的研究提示PaSC的活化也可能和胰腺癌的发生发展有关。我们的前期研究发现PaSC通过上皮-间质转化（EMT）而完成活化过程。近年来，《Cell》等权威杂志报道正常上皮细胞可以通过EMT而转变为恶性肿瘤细胞。为此，我们提出假设：PaSC通过EMT介导慢性胰腺炎向胰腺癌转变。并拟通过下列研究来进行验证，研究内容包括：正常PaSC的活化过程中EMT的发生以及干预EMT的发生后，PaSC的活化差异；体内种植荧光标记的PaSC和胰腺癌细胞株，观察PaSC的转归和EMT的发生；EMT关键信号通路干预后的标记PaSC再次种植，观察成瘤差异。通过上述研究，明确PaSC介导的EMT在慢性胰腺炎向胰腺癌的演变过程中的作用，也可以为慢性炎症和肿瘤的关系研究提供借鉴。

Chronic pancreatitis and pancreatic cancer are common disease. Chronic pancreatitis can develop to pancreatic cancer, However, its mechanism is unclear. Pancreatic stellate cells (PaSC) plays the most critical role in the development of chronic pancreatitis. Recent studies suggest that activation of PaSC may also be related to the initiation and progression of pancreatic cancer.Our previous studies found PaSC complete activation process by epithelial - mesenchymal transition (EMT).In recent years , that normal epithelial cells change into malignant cells by EMT process reported by Cell and Can Res.To this end, we propose hypothesis: PaSC mediated chronic pancreatitis developing to pancreatic cancer by EMT.And we intended to verify it by following researches: To investigate the occurrence of EMT in normal PaSC activation process and the changes of PaSC activation after intervention of EMT. Xenograft transplant fluorescently labeled PaSC and pancreatic cancer cell lines to observe the outcome of PaSC and the occurrence of EMT. Replant the labeled PaSC which key signaling pathway of EMT was intervented and to observe the differences in tumorigenicity. From the above research, function of EMT mediated by PaSC in evolution of chronic pancreatitis into pancreatic cancer would be confirmed, and a reference for exploration the relationship between chronic inflammation and cancer would be provided.