PCT在感染诊断和指导抗生素使用方面具有重要价值，是一种“理想”的感染生物标志物。但传统的PCT检测方法存在着检测设备复杂、操作繁琐、可视化程度低等问题。针对这一现状，本项目拟结合我们在多色荧光传感器的构建及可视化检测方面的工作基础，充分利用稀土纳米材料荧光颜色的可调性及色彩分析APP，建立一种快速便捷及可视化的PCT检测新方法。通过共价接枝法将两种不同荧光颜色的稀土纳米标记物与PCT单克隆配对抗体进行偶联，制备出PCT稀土纳米多色荧光探针。该探针根据PCT含量呈现不同的荧光颜色，可实现快速可视化检测，同时利用智能手机中的色彩分析APP可获取更准确、客观的检测结果。总结所获新知识及有关规律，为稀土纳米多色荧光探针在PCT快速可视化检测方面的应用提供新的思路和方法。

PCT is of great value in diagnosing and guiding the use of antibiotics and is an "ideal" infectious biomarker. However, the conventional detection methods have their drawbacks, such as complicated testing equipment and cumbersome operation. To fix this problem, this project intends to build a rapid and visual detection method for PCT, which is based on our previous work in the construction of multi-color fluorescence sensors and visual detection. Meanwhile, this project is also base on the tunability of fluorescent color and APP of color analysis of rare earth nanomaterials. Through the covalent grafting method, two kinds of rare earth nano-markers of different fluorescence colors were coupled with the monoclonal antibodies of PCT to prepare PCT rare earth nano-multi-color fluorescent probes. The probe exhibits different fluorescent colors based on PCT content for rapid visual inspection, while using the color analysis APP in smartphones for more accurate and objective test results. Summarizing the new knowledge gained and the related laws, we can provide new ideas and methods for the application of multi-color fluorescent rare earth nano-probes in rapid visual detection of PCT.