液相中自组装膜（SAM）表面氢键自组装和解组装的认识对理解分子识别有重要意义。本项目运用扫描电化学显微镜（SECM）方法研究液相中SAM表面氢键自组装和解组装中分子间氢键协同作用。通过设计使基底电极的SAM表面基团以分子间氢键键合探针分子，构筑包括分子间氢键解（自）组装的一个循环过程。此循环中，静电吸引（排斥）导致SAM表面基团解离平衡的移动，并诱导上述分子间氢键解（自）组装。借助解离平衡的调控实现远离基底产生持续的长程正（负）反馈电流。将SECM稳态极限扩散电流公式（iT.∞ = 4nFDCa）的应用范围扩展到长程正（负）反馈电流计算中，使实验上定量研究分子间氢键协同作用成为可能。SECM的优势是电子转移和氢键作用可分别发生在探针电极和基底电极上，避免了电子转移对氢键作用的干扰，同时基底电极的开路状态弱化了基底电场对氢键协同作用的干扰。实现液相中分子间氢键自（解）组装的原位电化学研究。

The understanding of hydrogen bonding self-assembly and disassembly on the surface of self-assembled monolayer (SAM) in liquid phase is important for understanding the molecular recognition. Here we aim to study the synergistic action of intermolecular hydrogen bond of self-assembly and disassembly on the surface of the SAM in liquid phase using SECM. A cyclic process including disassembly (self-assembly) of intermolecular hydrogen bond was constructed by designing a probe molecule that binds the surface group of the SAM substrate to an intermolecular hydrogen bond. In this cycle electrostatic attraction (repulsion) leads to the shift of dissociation equilibrium of the SAM surface group and induces the disassembly (self-assembly) of intermolecular hydrogen bond. Long-range positive (negative) feedback currents are generated away from the substrate base on the regulation of dissociation equilibrium. Then, the application range of the steady limit diffusion current formula (iT.∞ = 4nFDCa) is extended to the calculation of long range positive (negative) feedback current. It is possible to quantitatively study the synergistic action of intermolecular hydrogen bond in experiments. The advantage of SECM is that electron transfer and hydrogen bonding can occur on the probe electrode and substrate electrode, respectively, which avoid the interference of electron transfer on hydrogen bond. At the same time, the open-circuit state of the substrate electrode weakens the interference of the substrate electric field on the synergistic action of intermolecular hydrogen bond. The in situ electrochemical study of intermolecular hydrogen bond of self-assembly and disassembly in liquid phase is realized.