乌鳢水泡病毒(SHVV)感染导致我国乌鳢养殖业重大经济损失，目前还没有有效的防治措施。MicroRNA是一类非编码的小分子RNA，在病毒感染过程中发挥重要的调控作用。我们前期研究发现SHVV感染乌鳢细胞(SSN-1)导致细胞的microRNA-214 (miR-214)下调表达，而过表达miR-214类似物抑制SHVV的增殖。进一步从SHVV基因组搜索miR-214的靶基因发现N和P基因是miR-214的潜在靶基因。因此，本项目拟对miR-214在SHVV感染SSN-1细胞中的调控机制进行研究。主要内容包括：（1）在SHVV感染SSN-1细胞过程中，哪些病毒成分导致miR-214下调表达；（2）miR-214是否与潜在靶基因N和P的mRNA结合，降低N、P基因mRNA的含量，从而抑制SHVV的增殖。研究结果对认识miR-214如何调控SHVV的感染和研发新型抗SHVV的药物具有重要的意义。

Snakehead vesiculovirus (SHVV) infection has caused great economic loss to snakehead fish culture in China, and there are not any efficient preventive and therapeutic ways. MicroRNA is a class of small non-coding RNA that plays an important role in the regulation of virus infection. Snakehead vesiculovirus (SHVV) infection has caused great economic loss to snakehead fish culture in China, and there are not any efficient preventive and therapeutic ways. MicroRNA is a class of small non-coding RNA that plays an important role in the regulation of virus infection. Our previous research indicates that cellular microRNA-214 (miR-214) was down regulated in striped snakehead fish (SSN-1) cells upon SHVV infection, and overexpression of miR-214 mimic inhibited the replication of SHVV. Further target-gene prediction of miR-214 in SHVV genome revealed that N and P genes were the potential target genes of miR-214. Therefore, this project aims at illuminating the regulatory role of miR-214 in SSN-1 cells upon SHVV infection. The main studies included: (1) Identify viral components that are involved in the down regulation of miR-214 in SSN-1 cells during the infection of SHVV; (2) Investigate whether miR-214 decreases the concentration of mRNAs of N and P genes via binding to these mRNAs, thus resulting in the inhibition of SHVV replication. The obtained results are highly important to reveal how miR-214 regulates SHVV infection and develop novel drugs against SHVV infection.