本研究以太湖水华蓝藻微生物群落为研究对象，在申请人团队连续十年采集的太湖水华微囊藻群体样本及理化数据的基础上，进一步采集太湖水体中水柱的不同层面和一天中不同的时间的微生物组学样品，结合宏基因组学、宏转录组学、生态代谢组学研究手段进行微生物群体中C、N元素循环的微生物组学研究。通过分析微囊藻-附生细菌微群落中参与脂质代谢和毒素等次生代谢物质的合成、转运和降解相关的群体固有功能属性，研究微生物群体内部的C/N元素平衡的调节机理以及对环境因子的响应机制。本研究成果必将充实和完善水圈微生物参与碳、氮生物地球化学循环以及生态系统调节等方面研究的理论成果。同时，为研究湖泊生态系统中群落的固有特性、时空分布特征、环境限制因子、以及生态作用等方面提供研究基础，更进一步丰富湖泊生态学研究。

In this study, we will use Taihu Lake as the objects to investigate the mechanism of hydrosphere microbial communities involved in global carbon and nitrogen cycle from the aspects of metagenomic, ecology metabolism and environmental factors. The regulation mechanism of C/N element balance and the response mechanism to environmental factors in microbial population were studied by analyzing the intrinsic functional properties of the communities involved in the synthesis, transport and degradation of lipid metabolism and toxic secondary metabolites in micro-algae/epiphytic bacteria. The results of the study will enrich and improve the understanding of how hydrosphere microbial communities in the global biochemical cycle, acid rain, climate change and ecosystem regulation.