线粒体是维持植物细胞生命活动的重要能量细胞器，为植物细胞提供能量分子ATP和氨基酸及重要的植物激素，深入研究植物线粒体蛋白质稳态调控是植物学研究的重要方向。植物线粒体基因组编码约50种功能蛋白，目前对于植物线粒体编码基因的翻译调控机制研究还比较欠缺。我们前期工作发现线粒体Lon1相关蛋白ARM功能失活突变体具有增强的光胁迫耐受性表型，本项目将以此为切入点，采用分子生物学、生物化学、细胞生物学和蛋白质组学方法探明拟南芥ARM功能失活突变体耐光胁迫性状的生理和生化机理，研究ARM蛋白对线粒体编码基因翻译的调控和线粒体功能的影响，阐明ARM蛋白对线粒体蛋白质稳态和细胞内ROS水平的调控机制。本研究对线粒体蛋白质稳态调控基础研究具有重要的科学意义，ARM功能失活引起的耐强光胁迫性状具有很好的农业应用价值。

Plant cellular maintenance relies on the supply of ATP, amino acids and phytohormones from mitochondria. The investigation of factors that control mitochondrial proteostasis is an essential frontier of plant science. There are few studies that focus on the translational control of plant mitochondrion encoded genes. As a starting point, we found the light stress tolerance phenotype of a Lon1 associated ARM mutant. In this study, we aim to discover the mechanism of ARM loss-of-function mutant light stress tolerance, the role of ARM in mitochondrial translational control, and the links that bridge ARM and mitochondrial proteostasis and cellular ROS level. This innovative study plays an import part in mitochondrial proteostasis fundamental research and shows great potentials for further applications in agriculture.