幽门螺杆菌是一种寄生在胃粘膜上并能引发B型胃炎、溃疡以及胃癌的革兰氏阴性菌。该细菌合成了一种在细胞内占蛋白总量2%的富含组氨酸的小分子量蛋白质Hpn，我们课题组过去的研究工作发现该蛋白是幽门螺杆菌中一种重要的镍储运蛋白，部分被幽门螺杆菌分泌到体外，生成淀粉样纤维丝，是一种重要的毒理因子。Actin是真核细胞中一种重要的骨架蛋白，同时参与细胞分泌、吞噬、移动等过程，淀粉样蛋白tau通过与F-actin互作，影响到细胞骨架形态并且引起神经损伤。本研究课题着眼于利用荧光、紫外、ITC、停留等手段，研究幽门螺杆菌中淀粉样蛋白Hpn与actin互作的动力学与热力学，结合定点突变技术和核磁共振技术等研究关键氨基酸残基的作用；从细胞层面研究蛋白质之间的共定位，以及这种相互作用对细胞骨架及细胞的应激反应的影响，从而为幽门螺杆菌的致病机理研究提供重要线索。

Helicobacter pylori colonizes on the gastric epithelial cells and induces various kinds of gastric diseases including B-type gastritis, ulcer and cancer. This bacterium synthesizes a small histidine-rich protein Hpn accouting for up to 2 percent of total proteins synthesized. The previous work by our group indicates that this protein is an important nickel storage amyloid protein in Helicobacter pylori. Actin is one important cellular skeleton protein and is also involved in cellular secretion, cytosis and migration. Amyloid protein tau interacts with F-actin and in turn affect cellular skeleton and finally induces nerve damage. This project is focused on the interaction between the amyloid protein Hpn with.actin with various biophysical methods such as Fluorescence, UV, ITC and stopped flow. Supplemented with amino acid mutation and NMR, the key amino acids involved in protein-protein interaction are identified and verified. On the cell level, protein-protein colocalization and the effects on the cellular skeleton as well as the response from the gastric epithelial cells are investigated. The study will provide important clues for the pathological mechanisms of Helicobacter pylori.