芥子油苷是主要存在于十字花科植物中的次生代谢物质，具有重要的生物学功能，如参与植物的防卫反应和抗癌活性等。芥子油苷的代谢是一个非常复杂的过程，受到多种信号的精细调控，关于其代谢调控网络的研究已成为该领域中的一个研究热点。糖信号转导途径是研究得较为透彻的植物信号转导途径之一，它在植物环境适应性和能量中的作用，以及和植物激素等信号之间的互作研究较为深入，但糖信号转导途径对次生代谢物质的调节作用研究未见报道。我们前期研究表明糖处理能显著促进芥子油苷的生物合成与积累，并且发现糖信号转导元件参与对芥子油苷的调控。本项目以模式植物拟南芥为材料，进一步确定糖信号转导途径中参与调节芥子油苷生物合成的元件，以及这些元件通过MYB家族转录因子调控吲哚类和脂肪类芥子油苷的分子机制，结合遗传学分析阐明糖信号途径调控芥子油苷代谢的遗传途径与分子机理。为植物细胞信号之间的互作和芥子油苷的代谢调控网络研究提供理论依据。

Glucosinolates are a group of secondary metabolites mainly exist in cruciferae plants. Glucosinolates and their degradation products are known to play important roles in plant interaction with herbivores and micro-organisms. In addition, they are also important for human life because of the remarkable anti-carcinogenic activity of their hydrolysis products, isothiocyanates. The biosynthesis and metabolism of glucosinolates are a complex process, which is delicately modulated by multiple signaling molecules. Sugar signaling is one of the best elucidated plant signalling pathways,and its role in plant adaptation to environmental stresses and as source preferred by most organisms was well studied. However, little has been reported on the regulatory mechanism of sugar signal transduction pathway on metabolism of secondary metabolites. Our previous results indicate that glucose treatments significantly promoted the biosynthesis and accumulation of glucosinolates, and some components of sugar signalling pathway invovled in the regulation of glucosinolates biosynthesis. In the present study, mutants related to glucose signaling and glucosinolates biosynthesis will be used to further determine the components of sugar signaling pathway invovled in the regulation of glucosinolates biosynthesis, and their functions in regulating aliphatic and indole glucosinolate biosythesis by interaction with the transcriptional factors of MYB family. Combined with the genetic analysis, we will aim to elucidate the genetic pathway of the regulation of sugar siganaling on glucosinolates metabolism, and to clarify the molecular mechanism of this regulation. It will provide solid therotical basis for the study of cross-talk of plant signalings and metabolic regulation network of glucosinolates.