番茄不仅是一种重要的蔬菜作物，而且是研究果实发育和品质形成机制的模式体系。我们的前期研究发现，植物激素油菜素甾醇(brassinosteriod, BR) 通过其信号转导组分SlBZR1发挥促进番茄果实成熟和改善果实品质的作用，并利用ChIP-seq等技术鉴定到多个SlBZR1直接调控的品质性状相关靶基因，通过酵母文库筛选和iTRAQ蛋白组学技术相结合鉴定到数个SlBZR1直接调控的激素途径相关蛋白。本项目拟在此基础上，综合利用遗传学，细胞生物学，生物信息学和生物化学等多种技术手段，研究SlBZR1对品质性状相关基因的转录调控机制，以及作为与其他激素互作节点对果实品质的调控作用，以阐明SlBZR1及其互作网络调控番茄果实品质的分子机理，深化人们对果实发育和品质形成的激素互作调控机制的认识，并为通过化学调控和遗传工程的方法改善作物的品质提供理论依据和技术支撑。

Tomato is an important vegetable crop, as well as a perfect model system for study of fruit development and quality formation. Our previous surveys in tomato indicated that Brassinosteriod (BR) as kind of phytohormone accelerated fruit ripening and improved fruit qualities through BR signaling component SlBZR1. Several genes related to crucial quality charactristics were identified as direct targets of SlBZR1 and transcriptionally controlled by ChIP-seq technique. At the same time, phytohormone pathway related proteins interacting with SlBZR1 at protein level were identified by yeast two-hybrid screen and iTRAQ protemics technology. Based on above mentioned data, we will try to elucidate the regulation mechanism of SlBZR1 and its interacting network on tomato fruit quality by further investigating the transcriptional modulation of BZR1 on genes related to quality charactristics, as well as the regulation mechanism as an interaction nod of phytohormones in fruit quality formation by approaches of genetics, cell biology, bioinformatics and biochemistry. It will deepen our understanding of cross talk between phytohormones in modulating fruit development and quality formation, which will lay the theoretical and technical foundation for the improvement of the quality in crops through chemical regulation and metabolic engineering.