细胞受体是决定猪繁殖与呼吸综合征病毒(PRRSV)感染细胞关键因素。我们的研究表明，PRRSV感染的初期会明显促进细胞受体CD163及与CD163有相互作用的PRRSV新型细胞受体NMHC-IIA的表达，说明病毒感染过程中会对细胞受体表达进行调控，以进一步促进病毒的感染。为阐明PRRSV对细胞受体蛋白表达调控的分子机制，本项目拟在病毒易感细胞Marc-145中分别表达已确定对细胞具有调控功能的PRRSV Nsp1、Nsp2、Nsp11及N蛋白和与CD163结合的GP2a、GP4蛋白、与NMHC-IIA结合的GP5蛋白，明确对受体具有调控作用的关键蛋白。然后,利用超表达、RNA干扰及信号通路测定等手段获得PRRSV蛋白刺激产生的调控CD163/NMHC-IIA蛋白的关键调控因子，明确其调控信号通路。该研究成果将为更好地阐述PRRSV的致病机制提供依据，进而对疫病的防控提供新的思路和方法。

Virus-cell receptor is the key factor for porcine reproductive and respiratory syndrome virus (PRRSV) infection of host cells. Our study result proving that PRRSV cell receptors CD163 and NMHC-IIA, a new cell receptor interacted with CD163, were significantly up-regulated by PRRSV infection. It is indicated that to forming efficient infection, PRRSV will regulate the cell receptor expression level when virus infection of cells. For investigating the molecular regulation mechanism, this project intends to express the PRRSV proteins Nsp1, Nsp2, Nsp11 and N, which confirmed having regulation ablity to cells; GP2a and GP4 which interacted with CD163; GP5 which interacted with NMHC-IIA in Marc-145 cells respectively, to identify the key regulation protein of PRRSV to CD163/NMHC-IIA expression, and then overexpression, RNA interference and signal pathway analysis methods will be used to find the key regulatory factors, and then the regulation signaling pathways. The research results will better illustrate the pathogenic mechanisms of PRRSV, and provide new ideas and methods for PRRS epidemic prevention and control.