前期研究发现：大负荷运动可导致骨骼肌收缩蛋白和骨架蛋白发生降解，并对其降解机理进行了系统研究。作为骨骼肌细胞骨架之一的微管，在细胞器的定位、蛋白质合成、胞内物质运输、信号转导以及细胞运动等方面都扮演了重要角色。线粒体在细胞质中的分布和迁移往往与微管有关，线粒体常常排列成长链状，并与微管的分布相对应。近年研究发现，微管很可能对线粒体损害的关键环节具有重要作用。有关运动对骨骼肌微管的影响鲜有研究，而有关其影响机制，以及微管的变化对线粒体的结构和功能的影响及影响机制，尚未见到相关研究。.本项目拟通过动物实验观察大负荷运动对微管和线粒体的影响，通过细胞培养研究运动对微管的影响机制以及微管对线粒体结构和功能的调节机制，并在此基础上探究针刺是否可通过促进微管和线粒体的恢复，对运动导致的骨骼肌损伤发挥治疗作用。

Previous study has shown that: the movement of large loads can lead to degradation of contractile proteins and skeleton proteins in skeletal muscle, and the mechanism of degradation has been systematically studied. Microtubules, one of the skeletal muscle cytoskeletons, plays an important role in the positioning of organelles, protein synthesis, intracellular material transport, signal transduction and cell motility. Distribution and movement of mitochondrion in cytoplasm have relations with microtubules. Arrangement of Mitochondrion in cytoplasm corresponding microtubules. Microtubules play an important key roles in the damage of mitochondrion.The study of the effect of exercise on skeletal muscle microtubules has not been yet precisely clarified. However, no reports were yet available in the literature as to its mechanism, the effect of changed microtubule on mitochondrial structure and function and its mechanism. .In this study, we choose animal experiments in order to observe the effect of the movement of large loads on microtubules and mitochondria, establish the skeletal muscle cells culture model in order to research the effect mechanism of exercise on microtubules, as well as on mitochondrial structure and function of microtubule-regulating mechanism, and on this basis to explore whether acupuncture may play a therapeutic role on movement-caused skeletal muscle injury, by promoting the recovery of microtubules and mitochondria.