凡纳滨对虾Ca2+/Calmodulin信号通路在低盐逆境条件下的应答调节作用研究

可被淡化养殖是凡纳滨对虾（Litopenaeus vannamei）的重要经济性状。深入研究凡纳滨对虾在低盐条件下的渗透压调节分子机制，对于促进凡纳滨对虾淡化养殖及盐度抗逆育种工作具有重要意义。Ca2+/Calmodulin (Ca2+/CaM) 信号通路是逆境分子调控网络最重要的通路之一，本项目组通过对前期转录组数据分析发现，低盐条件下对虾Ca2+/CaM通路基因的表达量变化较大，预示着Ca2+/CaM通路可能在渗透压应答调节过程中起着重要的作用。本项目拟利用Ca2+/CaM通路拮抗剂和激活剂，研究低盐或低渗条件下Ca2+/CaM通路对重要的渗透压膜转运蛋白基因的应答调节作用，阐明凡纳滨对虾Ca2+/CaM通路在低盐逆境中的渗透压应答调节作用机制。通过本项目的研究，将建立凡纳滨对虾渗透压调节基因的互作机制研究模型，为进一步进行凡纳滨对虾耐低盐分子辅助育种工作提供理论指导。

The pattern of Pacific white shrimp (Litopenaeus vannamei) that can be cultured under low salinity or even fresh water has achieved great economic value. Much more researches on the molecular mechanism of the shrimp in response to low salinity stress are necessary. Ca2+/Calmodulin (Ca2+/CaM) signal pathway is one of the most important pathways to analyzing the stress response and adaption mechanism, which had been proposed in our previous researches as an important factor involved in regulating the low salinity stress response in Pacific white shrimp. In this project, Ca2+ signal pathway will be studied with the Ca2+ activator/antagonist in live and in vitro. The membrane transport protein genes will be the target genes relate to Ca2+/CaM pathway regulation. After this project, the molecular regulation mechanism of Ca2+/CaM pathway in response to low salt stress in Pacific white shrimp will be exposed and our researches will essentially promoting the shrimp breeding work.