猕猴桃细菌性溃疡病已成为目前世界猕猴桃生产中最具毁灭性的病害，对猕猴桃产量和品质造成重大损失。我们通过开展猕猴桃种质抗性筛选和转录组测序工作，发现NON-RACE SPECIFIC DISEASE RESISTANCE 1（NDR1）类似基因在猕猴桃抗病和感病种质中存在表达差异，并且有研究表明，NDR1基因在防御信号传导和植物抗病过程中发挥重要作用。本项目拟在已有研究工作的基础上，分离猕猴桃防御调控因子NDR1类似基因，采用实时定量分析其时空表达特异性及对溃疡病菌的响应，利用转基因技术研究NDR1类似基因的功能，揭示其在猕猴桃诱导抗病形成中的作用机制。并通过探索该类基因与上下游基因间的相互作用，进一步解析NDR1类似基因参与的调控网络途径。本研究的完成对阐明猕猴桃资源抗溃疡病的分子机理，指导溃疡病抗性种质的合理利用和抗性遗传改良具有重要的理论意义和实践价值。

Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), has become the most important destructive diseases in kiwifruit production worldwide. This results in serious losses to both yield and quality of kiwifruit. Here we carried out the resistance screening and transcriptome sequencing of kiwifruit germplasm resources, and found that there were differences in the expression of NDR1-like genes in resistant and susceptible germplasm. Previous studies have indicated that the NDR1 gene plays an important role in defense signal transduction and plant disease resistance. Based on our existing research works, we will isolate the defense regulator NDR1-like genes in kiwifruit, and analyze the spatial and temporal expression specificity of these genes and their response to Psa by real-time quantitative in this project. Transgenic analysis of over-expression and gene knockout will be carried out to reveal the mechanism of these genes in the induction of disease resistance of kiwifruit. The interaction between NDR1-like genes and their upstream and downstream genes will also be explored, aiming at analyzing the regulatory network of the NDR1-like genes involved in. Our research will provide important theoretical and practical value for elucidating the molecular mechanism of kiwifruit bacterial canker resistance, and guiding the rational use of disease resistant germplasm and genetic improvement of resistance.