肝移植急性排斥反应主要由T细胞介导,其机制尚不完全清楚。我们前期在大鼠肝移植急性排斥模型的研究中发现：CD8+T细胞凋亡与急性排斥反应程度呈负相关，抑制自噬能够促进CD8+T细胞凋亡从而减轻大鼠肝移植急性排斥反应。对人肝移植急性排斥反应肝穿刺标本检测显示CD8+T细胞自噬与排斥反应程度呈正相关。提示自噬能促进CD8+T细胞增殖和抑制其凋亡而介导肝移植急性排斥反应。既往研究证实JNK信号激活能够激活细胞自噬。我们在小鼠单向混合淋巴细胞培养实验也发现JNK信号可能通过诱导CD8+T细胞自噬而促进其增殖。我们由此推测：JNK信号可能通过上调自噬促进CD8+T细胞增殖而介导肝移植急性排斥反应。本项目拟用临床病例标本、基因敲除小鼠单向混合淋巴细胞培养和肝移植排斥反应模型验证这一科学问题，进一步揭示肝移植急性排斥反应机制。

The acute rejection after liver transplantation is mediated by T lymphocyte with uncertain mechenisms.Our previous studies on rat liver transplantion model show that the apoptosis of CD8+ T cells correlates negatively with the severity of the acute rejection after liver transplantation. Studies on liver tissues from the liver transplant patients with acute rejection also show that the autophagy of CD8+T cells correlate positively with the severity of the acute rejection after liver transplantation. Previous study showed that JNK can mediate autophagy.Our previous mouse unidirectional mixed lymphocyte culture experiment revealed that JNK signaling may promote proliferation of CD8+T cells by upregulating its autophagy. Thus, we speculate that JNK signaling mediates the acute rejection after liver transplantation by promoting CD8+ T cell proliferation through upregulating its autophagy.Here we are going to verify our speculation by using clinical samples, cell culture and acute rejection model of liver transplantation of gene knockout mice, and reveal furtherly the mechanisms of acute rejection after liver transplantation.